摘要
目的:研究黄芪和当归6种主要活性成分芒柄花素、黄芪皂苷Ⅰ、黄芪甲苷、毛蕊异黄酮、阿魏酸、毛蕊异黄酮苷单用及其配伍对大鼠血管平滑肌细胞(VSMCs)增殖与迁移的影响。方法:以血小板衍生生长因子BB(PDGF-BB)诱导VSMCs增殖,随机分为对照组、模型组、各成分单用组、各成分剔除组、六药配伍组。CCK-8检测VSMCs增殖,流式细胞术检测细胞周期,划痕实验检测细胞迁移能力,免疫荧光法检测增殖细胞核抗原(PCNA)、平滑肌α肌动蛋白(α-SMA)、骨桥蛋白(OPN)的表达。结果:各成分单用组(除芒柄花素单用组外)、各成分剔除组、六药配伍组均可显著抑制VSMCs增殖、细胞周期转化、PCNA的表达(P<0.05,P<0.01)。与六药配伍组比较,黄芪皂苷Ⅰ剔除组、黄芪甲苷剔除组、毛蕊异黄酮剔除组细胞增殖抑制率显著降低,增殖指数(PI)、PCNA蛋白表达显著升高(P<0.05,P<0.01);单用黄芪皂苷Ⅰ组、单用毛蕊异黄酮组、单用阿魏酸组、各成分剔除组、六药配伍组均可使细胞迁移降低,α-SMA表达上调,OPN蛋白表达下调(P<0.01),单用毛蕊异黄酮苷组可上调α-SMA表达(P<0.05)。与六药配伍组比较,黄芪皂苷Ⅰ剔除组、黄芪甲苷剔除组、毛蕊异黄酮剔除组均可使细胞迁移率增加,α-SMA表达降低,OPN表达增强(P<0.01);阿魏酸剔除组可使细胞迁移率和OPN表达增加(P<0.01)。结论:黄芪当归主要活性成分配伍对VSMCs表型转化、增殖和迁移的抑制作用强于药物单用,其中黄芪皂苷Ⅰ、黄芪甲苷、毛蕊异黄酮主要起抑制VSMCs增殖和迁移的作用,阿魏酸起抑制VSMCs迁移的作用,毛蕊异黄酮苷可抑制VSMCs增殖。黄芪和当归主要活性成分配伍的作用可能是通过抑制VSMCs表型转化和细胞周期进程而发挥的。
Objective: To study the effects of the compatibility of the six main active components of Astragalus membranaceus and Angelica sinensis, such as formononetin, astragaloside-Ⅰ, astragaloside Ⅳ, flavonoids, ferulic acid and flavonoid glycosides on the proliferation and migration of vascular smooth muscle cells(VSMCs) in rats. Methods: The proliferation of VSMCs was induced by platelet-derived growth factor(PDGF-BB). The cells were randomly divided into control group, model group, drug single-use groups, drug elimination groups, and six components combination group. The proliferation rate of VSMCs was detected by CCK-8 method, cell cycle was determined by flow cytometry, cell migration ability was detected by cell scratch test, the expression of proliferating cell nuclear antigen(PCNA), alpha-smooth muscle actin(α-SMA)and osteopontin(OPN) were detected by immunofluorescence assay. Results: Drug single-use groups(except for formononetin single-use group), drug elimination groups and six components combination group could significantly inhibit the proliferation of VSMCs, the transformation of cell cycle and the expression of PCNA(P<0.05, P<0.01). Compared with the six components combination group, the inhibition rate of cell proliferation in astragaloside Ⅰ elimination group, astragaloside Ⅳ elimination group and flavonoids elimination group decreased significantly, while the proliferation index(PI) and PCNA protein expression increased significantly(P<0.05, P<0.01). Astragaloside Ⅰ single-use group, flavonids single-use group, ferulic acid single-use group, drug elimination groups, six components combination group could all significantly inhibit cell migration, up-regulate the expression of α-SMA and down-regulate the expression of OPN protein(P<0.01). Flavonoids glycosides single-use group could up-regulate the expression of α-SMA(P<0.05). Compared with the six components combination group, astragaloside Ⅰelimination group, astragaloside Ⅳ elimination group and flavonoids elimination group could inc
作者
陈凌波
阎卉芳
邓常清
CHEN Ling-bo;YAN Hui-fang;DENG Chang-qing(Hunan University of Chinese Medicine,Changsha 410208,China)
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2022年第7期3811-3818,共8页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
国家自然科学基金面上项目(No.81874406)
湖南省教育厅科学研究项目(No.18B245)
湖南省研究生科研创新项目资助(No.CX2018B464)。
关键词
黄芪
当归
芒柄花素
黄芪皂苷Ⅰ
黄芪甲苷
毛蕊异黄酮
阿魏酸
毛蕊异黄酮苷
血管平滑肌细胞
Astragalus membranaceus
Angelica sinensis
Formononetin
Astragaloside Ⅰ
Astragaloside Ⅳ
Flavonids
Ferulic acid
Flavonoids glycosides
Vascular smooth muscle cells
