摘要
目的探讨帕金森病(Parkinson disease,PD)患者血浆磷酸化α突触核蛋白(phosphorylated α-synuclein,ps129-α-syn)与认知功能及运动症状的关系。方法招募2019年3月至2020年6月期间在河南省人民医院神经内科门诊和住院的PD患者90例为研究对象,并招募同时期来医院体检的认知功能正常的40名健康中老年人作为对照组,收集临床资料,采集血样标本,PD患者分为认知功能正常组(Parkinson disease normally cognitive,PD-NC)、轻度认知功能障碍组(mild cognitive impairment,PD-MCI)、和痴呆组(Parkinson disease dementia,PDD),采用酶联免疫吸附实验检测血浆磷酸化α突触核蛋白,采用相关分析评估PD患者血浆ps129-α-syn与病程、H-Y分期(Hoehn-Yahr,H-Y)、统一帕金森病评定量表(unified Parkinson's disease rating scale,UPDRS)、蒙特利尔认知评估量表(Montreal cognitive assessment,MoCA)、自主神经症状量表(the scale for outcomes in Parkinson's disease for autonomic symptoms,SCOPA-AUT)、汉密尔顿焦虑量表(14-item Hamilton anxiety rating scale,HAMA-14)、汉密尔顿抑郁量表(the 24-item Hamilton depression rating scale,HAMD-24)、左旋多巴每日等效剂量(levodopa equivalent daily dosage,LEDD)的关系,采用多重线性回归分析评估影响PD认知功能的因素。结果 PD患者血浆ps129-α-syn[(19.44±8.93)μg/L]较健康对照组[(10.78±5.87)μg/L]高(t=5.615,P<0.01),PD-MCI组[(19.64±7.77)μg/L]及PDD组患者[(23.79±9.47)ng/L]的ps129-α-syn均较PD-NC组[(13.37±5.40)μg/L]高(P<0.05)。相关分析显示PD患者ps129-α-syn与H-Y分期(r=0.404,P<0.01)、UPDRS-Ⅲ(r=0.275,P=0.009)、UPDRS-总分(r=0.211,P=0.046)、SCOPA-AUT呈正相关(r=0.335,P=0.001),与MoCA呈负相关(r=-0.459,P<0.01)。多重线性回归分析显示病程(t=-4.618,P<0.01)、ps129-α-syn(t=-3.792,P<0.01)及UPDRS-总分(t=-2.826,P=0.006)是影响PD认知功能的危险因素。PD患者血浆ps129-α-syn诊断PD认知正常组和PD认知障碍组的受试者工作特征曲线下面�
Objective To investigate the relationship between plasma phosphorylatedα-synuclein(ps129-α-syn)and cognitive function in Parkinson disease(PD).Methods This study recruited 90 PD patients who were hospitalized in the Department of Neurology,Henan province people's hospital from March 2019 to June 2020.Forty healthy middle-aged and elderly people with normal cognitive function who came to the hospital for physical examination were selected during the same period.Clinical characteristics and blood samples were collected.Patients with PD were classified into those with normally cognitive(PD-NC),mild cognitive impairment(PD-MCI),and dementia(PDD).The enzyme-linked immunosorbent assay was used to measure the plasma ps129-α-syn.Correlations between plasma ps129-α-syn and clinical characteristics such as disease duration,Hoehn-Yahr stage(H-Y),unified Parkinson's disease rating scale(UPDRS),Montreal cognitive assessment(MoCA),14-item Hamilton anxiety rating scale(HAMA-14),the 24-item Hamilton depression rating scale(HAMD-24),levodopa equivalent daily dosage(LEDD),the scale of outcomes in Parkinson's disease for autonomic symptoms,SCOPA-AUT)were analyzed using Pearson or Spearman correlation analysis.Multiple linear regression analysis was used to evaluate the factors affecting the cognitive function of PD.Results Plasma ps129-α-syn in PD patients was higher than that in healthy controls((19.44±8.93)μg/L,(10.78±5.87)μg/L,(t=5.615,P<0.01).Plasma ps129-α-syn was higher in PD-MCI group((19.64±7.77)μg/L)and PDD group((23.79±9.47)μg/L)compared with that in PD-NC group((13.37±5.40)μg/L)(P<0.05).Plasma ps129-α-syn was positively correlated with H-Y(r=0.404,P<0.01),UPDRS-Ⅲ(r=0.275,P=0.009),UPDRS-total(r=0.211,P=0.046)and SCOPA-AUT(r=0.335,P=0.001).Plasma ps129-α-syn was negatively associated with MoCA(r=-0.459,P<0.01).Multiple linear regression analysis suggested disease duration(t=-4.618,P<0.01),ps129-α-syn(t=-3.792,P<0.01)and UPDRS-total(t=-2.826,P=0.006)were independently associated with cognitive function.
作者
车宁宁
蒋秋焕
杨蓬勃
马建军
杨红旗
Che Ningning;Jiang Qiuhuan;Yang Pengbo;Ma Jianjun;Yang Hongqi(Department of Neurology,Henan Provincial People's Hospital,Zhengzhou 450000,China;Department of Clinical Medicine,Sanquan College,Xinxiang Medical College,Xinxiang 453003,China)
出处
《中华行为医学与脑科学杂志》
CAS
CSCD
北大核心
2022年第7期597-603,共7页
Chinese Journal of Behavioral Medicine and Brain Science
基金
河南省科技发展计划项目(192102310085)。
