摘要
A YEAST VPS SCREENING TO IDENTIFY Brucella PROTEINS THAT INTERFERE WITH MEMBRANE TRAFFICKING Brucella deliver effector proteins into host cells to interfere with cellular processes and promote infection.Identification and characterization of such proteins has been difficult,often limited by the lack of detectable signal sequences and functional redundancy.In this work,a pathogen effector protein screening in yeast was carried out identifying three B.abortus DNA fragments that produced vacuole protein sorting(VPS)defects in yeast.Cloning of the complete genes codified by these fragments independently demonstrated that htpX was responsible for the VPS defects in yeast.HtpX is a zinc metalloprotease homologue to S.cerevisiae STE24 involved in NH2-and COOH-terminal CAAX processing events of a-factor maturation.An htpX deletion mutant in B.abortus was constructed,showing a reduce virulence in mice in the chronic infection phase.
出处
《BIOCELL》
SCIE
2014年第S01期128-156,共29页
生物细胞（英文）
