摘要
目的通过体内实验研究散结消瘤方对乳腺癌小鼠移植瘤的影响,旨在探究散结消瘤方的疗效和作用机制。方法选取45只BaL B/c雌性小鼠建立乳腺癌小鼠移植瘤的模型,并随机分为五组,对照组(生理盐水灌胃)、散结消瘤方低、中、高剂量组(不同剂量散结消瘤方汤剂灌胃)、紫杉醇组(紫杉醇注射液腹腔注射),连续给药24 d,观察小鼠饮食和活动情况,记录移植瘤小鼠的体重、瘤体重量和移植瘤体积变化,计算抑瘤率。蛋白免疫印迹法(Western blot)检测各实验组的小鼠移植瘤组织中上皮间质转化相关蛋白(E-cadherin、N-cadherin、Vimentin)的表达。结果(1)经散结消瘤方干预的小鼠进食量和活动状态受乳腺癌移植瘤的影响较小。(2)给药组小鼠移植瘤瘤体的体积和重量均小于对照组(均P<0.01),且散结消瘤方中剂量组移植瘤体积小于散结消瘤方低剂量组(P<0.05)。各给药组之间的肿瘤抑制率分别为:散结消瘤方中剂量组52.4%,紫杉醇组40.3%,散结消瘤方低剂量组39.5%,散结消瘤方高剂量组34.1%。(3)Western blot结果显示,给药组小鼠移植瘤组织中E-cadherin表达水平高于对照组,N-cadherin、Vimentin表达水平低于对照组,且差异有统计学意义(均P<0.05)。结论散结消瘤方可以改善乳腺癌小鼠的虚弱、进食量减少等表现,在一定程度上抑制小鼠移植瘤生长,其机制可能是散结消瘤方可以抑制乳腺癌的上皮间质转化,抑制乳腺癌侵袭和转移的发生。
Objective To study the effect of Sanjie Xiaoliu Recipe on transplanted tumors in breast cancer mice through in vivo experiments,in order to explore the efficacy and mechanism of Sanjie Xiaoliu Recipe on breast cancer patients.Methods 45 BaL B/c female mice were selected to establish the transplanted tumor model of breast cancer.All the transplanted tumor models of breast cancer mice were randomly divided into five groups:the control group(intragastric administration of normal saline),the low,medium and high dose group of Sanjie Xiaoliu Recipe(intragastric administration of different doses of Sanjie Xiaoliu Decoction),and the paclitaxel group(intraperitoneal injection of paclitaxel).After 24 days of continuous administration,the diet and activities of mice were observed;the body weight,weight and volume changes of transplanted tumor mice were recorded,and the tumor inhibition rate was calculated.Western blot was used to detect the expression of epithelial mesenchymal transformation related proteins(E-cadherin,N-cadherin,Vimentin)in the transplanted tumor tissues of mice in each group.Results(1)The food intake and activity status of mice treated with Sanjie Xiaoliu Recipe were less affected by the transplanted tumor of breast cancer.(2)The volume and weight of transplanted tumor in the treat groups were smaller than those in the control group(all P<0.01),and the volume of transplanted tumor in the middle dose group of Sanjie Xiaoliu Recipe was smaller than that in the low dose group(P<0.05).The tumor inhibition rates among the treatment groups were:Sanjie Xiaoliu Recipe medium dose group 52.4%,paclitaxel group 40.3%,Sanjie Xiaoliu Recipe low dose group 39.5%,Sanjie Xiaoliu Recipe high dose group 34.1%.(3)The results of Western blot showed that the expression level of E-cadherin in the transplanted tumor tissue of the treat groups was higher than that in the control group,and the expression levels of N-cadherin and Vimentin were lower than that in the control group,with statistically significant difference(all P<0.0
作者
何东燕
吴玉华
陈茂
彭哲
廖宇杉
欧阳震文
胡伟佳
He Dongyan;Wu Yuhua;Chen Mao;Peng Zhe;Liao Yushan;Ouyang Zhenwen;Hu Weijia(Gradute School of Hunan University of Chinese Medicine,Changsha 410208,China;Department of Oncology,Hunan Academy of Traditional Chinese Medicine Affiliated Hospital,Changsha 410006,China)
出处
《中国医师杂志》
CAS
2022年第7期996-1001,共6页
Journal of Chinese Physician
基金
湖南省科技创新计划项目(2018sk50801)。
关键词
乳腺肿瘤
抗肿瘤药(中药)
散结消瘤方
上皮-间质转化
Breast neoplasms
Antineoplastic drugs(TCD)
Sanjie anti-tumor formula
Epithelial-mesenchymal transition
