摘要
目的研究轻型颅脑爆炸冲击伤后大鼠不同时间脑组织星形胶质细胞、小胶质细胞和神经元损伤反应的病理改变和过程。同时探讨富含鱼油饮食对轻型颅脑爆炸冲击伤大鼠的神经保护作用。方法收集54只刚断乳SD大鼠,随机均分为对照组、模型组、治疗组,模型组及治疗组大鼠分别在喂养普通饲料和富含鱼油的饲料33 d后建立由冲击波诱导的轻型颅脑爆炸冲击损伤模型,对照组大鼠在喂养普通饲料33 d后不建立爆炸冲击波损伤模型。结果与对照组相比,模型组和治疗组的大鼠体质量在致伤后出现一定程度的减轻,随后恢复至致伤前水平;轻型爆炸伤后6 h、24 h、3 d,模型组和治疗组大鼠大脑海马区GFAP阳性染色星形胶质细胞数量及海马区锥体细胞数量均减少。反之,模型组和治疗组大鼠脑组织中海马区的激活态小胶质细胞和皮层区域凋亡神经元数量均增加,且具有时间依赖性。此外,与模型组相比,治疗组可增加伤后星形胶质细胞和海马区锥体细胞数量,并降低激活态小胶质细胞和皮层区域凋亡神经元数量。结论富含鱼油饮食可通过减轻颅脑爆炸伤后神经细胞损伤,抑制神经炎性反应等方面发挥其神经保护作用。
Objective To study the neuropathological changes and process brain astrocyte microglia and neuron injury in rats at different time after mild blast-related traumatic brain injury(bTBI),and to investigate the neuroprotective potential of dietary supplementation with fish oil on mild bTBI rats.Methods 54 newly weaned SD rats were randomly divided into control group(n=18),model group(n=18)and treatment group(n=18).The model group and treatment group were fed with ordinary diet and oil-rich diet for 33 days to establish the mild bTBI model by shock wave,respectively.The control group rats were fed with ordinary diet without shock wave injury.Results Compared with control group,the weight of rats in the model group and treatment group decreased to a certain extent within 2 days after injury,and then recovered to the level before injury.After bTBI injury for 6 h,24 h and 3 d,the number of GFAP positive staining astrocytes and vertebral cells in hippocampal area decreased in both model group and treatment group,whereas the number of activated microglia and apoptotic neurons in the hippocampus increased in a time-dependent manner in model group and treatment group.In addition,compared with the model group,the treatment group with oil-rich diet increased the number of astrocytes and pyramidal cells in the hippocampus after injury,and decreased the number of activated microglia and apoptotic neurons in the lesions area.Conclusion Pre-injury dietary supplementation with fish oil shows neuroprotective benefits in alleviating neurons injury and inhibiting neuroinflammatory response in a rat model of mild bTBI.
作者
张雯倩
王涛
王佳
王培新
顾建文
赵全军
Zhang Wenqian;Wang Tao;Wang Jia;Wang Peixin;Gu Jianwen;Zhao Quanjun(306^(th) Clinical College of PLA,Fifth Clinical Medical College,Anhui Medical University,Beijing 100101;Dept of Neurosurgery,Strategic Support Force Specialized Medical Center,Beijing 100101;Dept of Neurology,Shangluo Central Hospital,Shanxi 726000)
出处
《安徽医科大学学报》
CAS
北大核心
2022年第8期1200-1205,共6页
Acta Universitatis Medicinalis Anhui
基金
国家自然科学基金(编号:31770386)
中心级课题(编号:19ZX11)。
