摘要
目的比较精神分裂症患者不同时期肠道菌群与细胞因子水平的变化,分析其与疾病症状之间的相关性,探索精神分裂症患者肠道菌群的变化对分裂症患者症状的影响。方法纳入住院分裂症患者不同时期各40例,收集病程资料、性别、年龄、体质指数(BMI)等资料,收集血清,用超敏电化学发光分析技术检测患者细胞因子的水平,使用阳性与阴性症状量表(PANSS)评估分裂症患者的病情严重程度,采用MetaGeneMark进行患者肠道菌群的宏基因测序。结果患者急性期血清白细胞介素-6(IL-6)、白细胞介素-10(IL-10)、白细胞介素-17(IL-17)、白细胞介素-23(IL-23)和肿瘤坏死因子-α(TNF-α)水平均高于缓解期,两者结果有统计学的意义(P<0.05)。阳性与阴性症状量表(PANSS)阳性因子分与TNF-α和IL-17呈负相关(r=-0.323,P<0.05;r=-0.356,P<0.05);IL-23与量表阴性因子得分显示正相关(r=-0.349,P<0.05);IL-6与量表认知因子得分显示正相关(r=-0.388,P<0.05);IL-23与量表总分呈正相关(r=-0.368,P<0.05)。急性期分裂症患者粪便样本内检测出的微生物多样性低于缓解期分裂症微生物的多样性,急性期与缓解期患者肠道菌群物种组成存在一定差异。结论精神分裂症急性期血清细胞因子水平高于缓解期,部分细胞因子水平与临床症状存在相关性,患者的不同时期肠道微生物物种组成及多样性均发生了改变。
Objective This study is designed to compare the changes of intestinal microflora and cytokine levels in patients with schizophrenia in different periods, analyze the correlation between intestinal microflora and disease symptoms, and explore the influence of intestinal microflora changes on the symptoms of patients with schizophrenia. Methods 40 schizophrenic patients in different periods were included in the study, with their demographic data of age, body mass index(BMI), sex and course of disease collected. For each subject, serum was first collected for the levels of cytokines to be determined by an Meso Scale Discovery. The severity of schizophrenia was then assessed using negative and positive symptom scales. Finally macrofactor sequencing of intestinal flora was performed using MetaGeneMark. Results Serum levels of interleukin-6(IL-6), interleukin-10(IL-10), interleukin-17(IL-17), interleukin-23(IL-23), and tumor necrosis factor-α(TNF-α) in the acute stage were higher than those in remission stage, and the results were statistically significant(P<0.05). Negative and positive symptom Scale(PANSS) positive factor scores were negatively correlated with TNF-α and IL-17(R=-0.312, P<0.05;R=-0.399,P<0.05);IL-23 was positively correlated with the negative factor score of the scale(R=-0.344, P<0.05). IL-6 was positively correlated with scale cognitive factor score(R=-0.339, P<0.05). IL-23 was positively correlated with the total score of the scale(R=-0.370, P<0.05). The microbial diversity detected in stool samples of patients with acute schizophrenia was lower than that of patients with remission, and certain difference was detected in the intestinal flora species composition between patients in the acute stage and in the remission stage. Conclusion The level of serum cytokines in the acute stage of schizophrenia is higher than that in the remission stage, and some cytokines levels are correlated with clinical symptoms.
作者
郜见亮
张爱国
姚理慧
陈苑苑
程鹏
苏虹
张许来
Gao Jianliang;Zhang Aiguo;Yao Lihui;Chen Yuanyuan;Cheng Peng;Su Hong;Zhang Xulai(Dept of Psychiatry,Affiliated Psychological Hospital of Anhui Medical University,Hefei 230022;Anhui Mental Health Center,Anhui Clinical Research Center for Mental Disorders,Hefei 230022;Dept of Epidemiology and Health Statistics,School of Public Health,Anhui Medical University,Hefei 230022)
出处
《安徽医科大学学报》
CAS
北大核心
2022年第7期1142-1146,共5页
Acta Universitatis Medicinalis Anhui
基金
国家自然科学基金(编号:81773518)。
