摘要
目的探讨阿霉素肾病综合征模型大鼠的潴留钠存储位置及其发生机制。方法采用两次尾静脉注射阿霉素(第1周4 mg/kg、第2周2 mg/kg)方法建立肾病综合征模型。检测尿液及血液生化指标;干燥法检测大鼠皮肤含水量;原子吸收光谱法检测皮肤钠含量;ELISA法检测糖胺聚糖含量;Western blot检测肾皮质张力反应性增强因子结合蛋白(TonEBP),血管内皮生长因子C(VEGFC),血管内皮生长因子受体3(VEGFR3),淋巴管内皮细胞受体-1(LYVE-1)表达;免疫荧光法检测肾皮质淋巴管分布。结果模型组大鼠尿蛋白排泄增高(P<0.05或0.01),且肾功能、血脂指标异常,提示模型建立成功。尿Na+排泄下降(P<0.05或0.01),皮肤钠含量(P<0.01)、糖胺聚糖含量升高(P<0.01),肾皮质TonEBP,VEGFC,VEGFR3,LYVE-1蛋白表达升高(P<0.05或0.01),肾皮质淋巴管密度增加(P<0.01)。结论肾病综合征大鼠机体发生钠潴留,且潴留的钠存储在皮肤的机制可能与TonEBP/VEGFC/VEGFR3有关。
Objective To investigate the locations of sodium retention and the mechanism of its occurrence in the rat model of adriamycin nephrotic syndrome. Methods The nephrotic syndrome model was established by tail vein injections of adriamycin twice two weeks(4 mg/kg in first week and 2 mg/kg in second week). Urine and blood biochemical parameters were detected;the contents of water and sodium in rat skin were detected respectively by desiccation and atomic absorption spectrometry;the content of glycosaminoglycan was analyzed by ELISA;the expression of tonicity-responsive enhancer binding protein(TonEBP), vascular endothelial growth factor C(VEGFC), vascular endothelial growth factor receptor 3(VEGFR3) and lymphatic vessel hyaluronan receptor-1(LYVE-1) in the renal cortical was detected by Western blot;the lymphatic vessels distribution in the renal cortex was measured by immunofluorescence. Results the model rats had increased urinary protein excretion, and abnormal renal function and lipid, which suggested the model was successfully established. The excretion of Nain urine decreased, the content of skin sodium and glycosaminoglycan in skin obviously increased, the expression of TonEBP, VEGFC, VEGFR3, LYVE-1 in renal cortical markedly increased, and density of lymphatic vessels in renal cortical notably increased(P<0.05 or P<0.01). Conclusion Sodium retained in nephrotic syndrome rats may be stored in the skin, which is related to the TonEBP/VEGFC/VEGFR3 pathway.
作者
倪良厚
杨沫
李曼曼
王运来
宣自华
许钒
Ni Lianghou;Yang Mo;Li Manman;Wang Yunlai;Xuan Zihua;Xu Fan(College of Pharmacy,Anhui University of Chinese Medicine,Hefei 230012;Key Laboratory of Chinese Medicine Formula of Anhui Province,Hefei 230012)
出处
《安徽医科大学学报》
CAS
北大核心
2022年第7期1054-1059,共6页
Acta Universitatis Medicinalis Anhui
基金
国家自然科学基金(编号:81974536、81573720)。
关键词
肾病综合征
钠潴留
淋巴
皮肤
钠离子
nephrotic syndrome
sodium retention
lymphatic
skin
sodium
