摘要
目的 探讨不同的取卵与ICSI注射(OPU-ICSI)时间间隔对ICSI周期临床结局的影响。方法 回顾性分析2015年9月至2020年5月在中山大学附属第三医院生殖医学中心因男性因素不孕而接受ICSI治疗的1 205例患者的临床资料。按OPU-ICSI时间间隔不同分为两组:间隔2~4 h者纳入A组(362例),间隔>4 h者纳入B组(843例)。比较两组患者的一般情况、实验室指标和临床结局。结果 两组患者的一般情况、注射HCG与取卵(HCG-OPU)时间间隔及不同卵巢刺激方案比例均无显著性差异(P>0.05);两组患者的卵子成熟率、受精率、2PN受精率、异常受精率、优胚率等实验室指标均无显著性差异(P>0.05);两组患者的临床妊娠率、流产率、活产率及新生儿平均出生体重亦无显著性差异(P>0.05)。Pearson相关性分析结果表明,OPU-ICSI时间间隔和卵子成熟率、2PN受精率及优胚率均无显著相关性(P>0.05)。二元Logistic回归分析结果表明,女方年龄、获卵总数、2PN受精率和优胚率能显著影响临床妊娠率(P<0.05),而OPU-ICSI时间间隔等与临床妊娠率没有显著关联(P>0.05)。结论 OPU-ICSI时间间隔并不影响ICSI周期的实验室指标和临床结局,临床工作中可以灵活安排ICSI注射时间,以提高实验室工作效率及优化工作流程。
Objective:To investigate the effect of time interval between oocyte pick-up and ICSI injection(OPU-ICSI) on clinical outcome in ICSI cycle.Methods:The cycle data of 1 205 patients who received ICSI treatment for male factor infertility in the Reproductive Medicine Center of the Third Affiliated Hospital of Sun Yat-sen University from September 2015 to May 2020 were retrospectively analyzed. According to the OPU-ICSI time interval, the patients were divided into two groups: the time interval was 2-4 hours in group A and >4 hours in group B. The general status, laboratory indicators and clinical outcomes of the two groups were compared.Results:There were no significant difference in general status, time interval between HCG injection and oocyte pick-up(HCG-OPU) and the proportion of different ovarian stimulation protocols between the two groups(P>0.05). There were no significant differences in the oocyte maturation rate, fertilization rate, 2 PN fertilization rate, abnormal fertilization rate, high-quality embryo rate and other laboratory indicators between the two groups(P>0.05). There were also no significant differences in the clinical pregnancy rate, abortion rate, live birth rate and average birth weight of newborn between the two groups(P>0.05). Pearson correlation analysis showed that there was no significant correlation between OPU-ICSI time interval and oocytes maturation rate, 2 PN fertilization rate and high-quality embryo rate(P>0.05). The results of binary regression analysis showed that female age, total number of oocyte pick-up, 2 PN fertilization rate and high-quality embryo rate significantly affected the pregnancy rate(P<0.05). However, there was no significant correlation between OPU-ICSI interval and clinical pregnancy rate(P>0.05).Conclusions:OPU-ICSI time interval does not affect the laboratory indicators and clinical outcome in ICSI cycle. ICSI injection time can be flexibly arranged in clinical work to improve laboratory efficiency and optimize workflow.
作者
宏苹苹
李涛
欧建平
HONG Ping-ping;LI Tao;OU Jian-ping(Reproductive Medicine Center,the Third Affiliated Hospital of Sun Yat-sen University,Guangzhou510630)
出处
《生殖医学杂志》
CAS
2022年第8期1049-1054,共6页
Journal of Reproductive Medicine
