摘要
以西藏昌都市高海拔地区青稞种植农田土壤为材料,采用4℃低温培养手段,从R和M5培养基中分离得到两株根瘤菌,菌株编号为T786和T808。经形态学、生理生化鉴定和16S rRNA序列分析,初步鉴定为Neorhizobium属和Pararhizobium属根瘤菌;进一步对菌株的ATP操纵子(atpD)、重组蛋白A(recA)、RNA聚合酶β亚基(rpoB)、苏氨酸合酶(thrC)和谷氨酰胺合成酶(glnII和glnA)共6对管家基因序列进行分析,发现菌株Neorhizobium sp.T786和Pararhizobium sp.T808的6对管家基因相似度和多基因ANI值均远小于根瘤菌新种阈值(95%~96%),表明菌株T786和T808为潜在的根瘤菌新种。菌株T786和T808的发现对丰富青藏高原地区根瘤菌种质资源具有重要意义。
Two rhizobia strains,named T786 and T808,were isolated from the highland barley cultivation soil in Qamdo,Tibet,using R and M5 media at 4℃,respectively.After morphological,physiological,biochemical identification and 16S rRNA sequence analysis,strains T786 and T808 were preliminarily identified to genus Neorhizobium and Pararhizobium.The multiple sequence alignment results of 6 pairs housekeeping genes(atpD,recA,rpoB,thrC,glnII and glnA)in T786 and T808 showed that the similarity of these housekeeping genes and ANI values in strains T786 and T808 were lower than in the threshold of new species of rhizobia(95%-96%).Strains T786 and T808 were potential new species of rhizobia,the discovery of strains T786 and T808 was significance for enriching the germplasm resources of rhizobia in Qinghai-Tibet Plateau.
作者
周洁
周子琼
潘虎
卢向阳
张一帆
田云
ZHOU Jie;ZHOU Ziqiong;PAN Hu;LU Xiangyang;ZHANG Yifan;TIAN Yun(College of Bioscience and Biotechnology,Hunan Agricultural University,Changsha 410128,China;Institute of Agricultural Product Quality Standard and Testing Research,Tibet Academy of Agricultural and Animal Husbandry Sciences,Tibet 850032,China;School of Food Science,Tibet Institute of Agriculture and Animal Husbandry,Linzhi 860000,China)
出处
《生物学杂志》
CAS
CSCD
北大核心
2022年第4期24-27,32,共5页
Journal of Biology
基金
国家自然科学基金项目(32060025)
西藏自治区自然科学基金项目(XZ202101ZR0090G)
湖南省战略性新兴产业科技攻关与重大科技成果转化项目(2019GK4018)
湖南农业大学“双一流”建设项目(SYL201802002)。
关键词
根瘤菌
管家基因
鉴定
潜在新种
rhizobia
housekeeping gene
identification
potential new species
