摘要
为保障进出口食品添加剂的安全,提升食品添加剂红曲红色素中有害物质桔青霉素的筛查效率,本文通过比较不同前处理方法对红曲红色素中桔青霉素含量测定的影响,对高效液相色谱法检测红曲红色素中桔青霉素的方法进行优化并建立质谱法进行确证。用80%的甲醇-水溶液作为提取剂,经HLB固相萃取小柱净化,用高效液相色谱仪配荧光检测器测定桔青霉素的含量。本方法检出限为3μg/kg,在浓度10~500μg/mL范围内标准曲线线性关系良好(r=0.9998);在10~100μg/kg添加水平下,回收率为83.6%~94.7%,相对标准偏差为2.85%~3.42%。并应用此方法对市面销售红曲红色素样品进行分析,该方法检测时间短、检测成本低、定量准确且可操作性强,适用于红曲红色素及其制品中桔青霉素的定性定量检测。
In order to ensure the safety of imported and exported food additives,improve the screening efficiency of citrinin in food additive Monascus red pigment.The influence of different pretreatment methods on the determination of citrinin in monascus red pigment was compared,and the method for detecting citrinin in monascus red pigment by High Performance Liquid Chromatography was optimized.Using 80%methanol-aqueous solution as extractant,the content of citrinin in monascus red pigment was purified by HLB solid phase extraction cartridge,determined by High Performance Liquid Chromatography with fluorescence detector,then the HPLC-MS/MS was used to do confirmation.The detection limit of this method was 3μg/kg.The standard curve obtained in the concentration range of 10~500μg/mL had a good linear relationship(r=0.9998);at the addition level of 10~100μg/kg,the recovery rate of standard addition was between 83.6%and 94.7%,the relative standard deviation was between 2.85%and 3.42%.This method was used to analyze and test monascus red pigment samples sold in the market.This method has short detection time,lower detection cost,more accurate quantification and stronger operability.It is suitable for the qualitative and quantitative detection of citrinin in monascus red pigment and its products.
作者
彭碧宁
余创波
曾川
冯强
刘勇
潘亮
PENG Bining;YU Chuangbo;ZENG Chuan;FENG Qiang;LIU Yong;PAN Liang(Gongbei Customs Technology Center,Zhuhai 519000;Zhuhai Golden Land Natural Colours Co.,Ltd.,Zhuhai 519045)
出处
《中国食品添加剂》
CAS
北大核心
2022年第8期208-215,共8页
China Food Additives
基金
珠海进出口公共技术服务平台产学研协同创新计划项目(IETP202001014)。
