摘要
MYB转录因子是植物中最大的一类转录因子家族,MYB蛋白在抵抗非生物胁迫调控中有重要作用.基于玉米MYB转录因子家族成员,对其功能进行分析,以揭示玉米MYB转录因子家族蛋白应答干旱胁迫的功能研究.从PlantTFDB数据库获取玉米MYB转录因子的氨基酸序列以及蛋白质理化性质,利用Pfam数据库、ProtComp、SOPMA、SWISS-MODEL、MEME、MEGA11.0、TBtools等软件进行结构域识别、亚细胞定位、蛋白质二级及三级结构预测、系统进化树构建.从PlantTFDB中获取203个玉米MYB转录因子成员,按成员MYB结构域重复数目的不同,将其分为五类:MYB-related、R2R3-MYB、3R-MYB、4R-MYB及5R-MYB.理化性质分析表明,玉米MYB蛋白编码188~1060个氨基酸;相对分子质量在20693.7~117692 kD之间;等电点在4.2977~12.2974之间.根据已知功能的筛选,玉米MYB成员中具有抗旱功能的蛋白有16个,结构以无规则卷曲为主.未知功能的玉米MYB蛋白与拟南芥构建进化树,预测有48个玉米MYB蛋白与拟南芥抗旱蛋白亲缘关系较近,表明这48个MYB蛋白可能与玉米抵抗干旱胁迫功能有关.数据库中获取的203个玉米MYB转录因子成员具有抗旱功能的有16个,结构进化保守,组织表达模式多样.根据功能预测的结果可知,有48个未知功能的MYB转录因子可能与玉米抵抗干旱胁迫相关.
MYB transcription factors are the largest family of transcription factors in plants.MYB proteins play an important role in resisting abiotic stress.Based on the members of maize MYB transcription factor family,their functions were analyzed in order to reveal the function of maize MYB transcription factor family proteins in response to drought stress.The amino acid sequence and protein physicochemical properties of maize MYB transcription factor were obtained from PlantTFDB database,the domain was identified by Pfam database,and the phylogenetic tree was constructed by MEGA11.0 software.Conservative motif analysis was carried out by MEME program,and TBtools software was used to analyze and visualize the data.The subcellular localization of MYB protein was carried out by using ProtComp online software.The secondary and tertiary structures of the protein were predicted by SOPMA and SWISS-MODEL online software.For MYB transcription factors with unknown functions,homologous sequence alignment was carried out with MYB transcription factors involved in abiotic stress regulation in Arabidopsis to construct evolutionary tree,predict function,and then predict MYB protein with drought resistance function.203 maize MYB transcription factor members were obtained from PlantTFDB and divided into five groups according to the repeat number of MYB domains:MYB-related,R2R3-MYB,3R-MYB,4R-MYB and 5R-MYB.The analysis of physical and chemical properties showed that maize MYB protein encodes 188—1060 amino acids,the molecular weight of maize MYB protein family is between 20693.7 and 117692 kD,and the isoelectric point of corn MYB protein family is between 4.2977 and 12.2974.According to the screening of known functions,there are 16 drought-resistant proteins in maize MYB members,and the conserved motifs and domains of drought-resistant proteins are similar.The prediction results of tertiary structure showed that the structure of drought resistant protein of maize MYB transcription factor was close to the predicted structure of seconda
作者
周晓馥
刘美琦
金妍
徐洪伟
ZHOU Xiao-fu;LIU Mei-qi;JIN Yan;XU Hong-wei(Collage of Life Science,Jilin Normal University,Siping 136000,China;Jilin Provincial Key Laboratory of Plant Resource Science and Green Production,Jilin Normal University,Siping 136000,China)
出处
《吉林师范大学学报（自然科学版）》
2022年第3期100-111,共12页
Journal of Jilin Normal University:Natural Science Edition
基金
吉林省科技发展计划项目(20190301059NY)
中央指导地方科技发展资金吉林省基础研究专项资助项目(202002016JC)。
关键词
玉米
MYB转录因子
干旱胁迫
功能研究
Zea mays
MYB transcription factor
drought stress
functional study
