摘要
目的探讨1型固有淋巴细胞(LCI)对肠源性脓毒症免疫抑制发生的预测价值。方法前瞻性分析2020年8月至2021年8月收治的20例因结肠穿孔所致肠源性脓毒症患者的临床资料,并在入院24h内收集其外周血,根据是否伴有免疫抑制分为两组(不伴有免疫抑制组10例和伴有免疫抑制组10例)。应用SPSS22.0进行统计学分析,连续变量资料例如年龄、BMI及外周血中淋巴细胞计数、各类固有淋巴细胞比例等采用(x±s)表示,组间比较采用独立样本t检验;非正态分布资料采用Mann-Whitney U检验,卡方检验和Fisher检验用以比较患者性别构成比。P<0.05为差异有统计学意义。结果两组患者在平均年龄、性别构成及BMI方面均具有可比性,差异无统计学意义。伴有免疫抑制的肠源性脓毒症患者,其淋巴细胞计数显著低于不伴有免疫抑制的脓毒症患者[(1.6±0.4)vs.(0.6±0.3),P<0.01];ILC1占所有固有淋巴细胞的比例,在伴有免疫抑制的肠源性脓毒症患者外周血中显著上调[(27.3±6.3)vs.(45.4±6.7),P<0.0001]。通过构建ROC曲线,发现当ILC1占所有固有淋巴细胞比例>32.5%时,其对预测肠源性脓毒症免疫抑制的发生具有较好的敏感性(90.9%)和特异性(81.8%)。结论检测所有固有淋巴细胞中1型固有淋巴细胞占比能够较好的预测肠源性脓毒症免疫抑制的发生,具有预测价值。
Objective To investigate the predictive value of group 1 innate lymphocyte(ILC1)in the occurence of immunosuppression in intestinal sepsis.Methods The clinical data of 20 patients with intestinal sepsis caused by colon perforation from August 2020 to August 2021 were prospectively analyzed and peripheral blood were dropped within 24 hours of admission.Two groups were set according to whether patients were accompanied by immunosuppression(10 cases in the group w ithout immunosuppression vs.10 cases in the group with immunosuppression).SPSS 22.0 was used for statistical analysis.Continuous variable data such as age,BMI,lymphocytes Counts and various types of intinsic lymphoeyte ratios are expressed by(x±s),Independent sample t test was used for comparison between groups.Non-normally distributed data were compared by mann-Whiney U test,Chi-square test and Fisher test.P<0.05 is considered staistically significant.Results The iwo groups of patients were comparable in terms of average age,gender composition and BMI,and the difference was not statistically significant.The lymphocyte count of patients with intestinal sepsis accompanied by immunosuppression was significantly lower than that of patients without immunosuppession[(1.6±0.4)vs.(0.6±0.3),P<0.01].The proportion of ILC1 innate lymphoid cells among all innate lymphoid cells was signifcantly up-regulated in the peripheral blood of patients with intestinal sepsis accompanied by immunosuppression[(27.3±6.3)vs.(45.4±6.7),P<0.0001].By constructing ROC curve,it is found that ILC1 had good sensitivity(90.9%)and specificity(81.8%)for predicting the occurrence of intestinal sepsis immunosuppression when the proportion of all inherent lymphocytes was>32.5%.Conclusion The proportion of group 1 innate lymphocytes in all lymphocytes can better predict the occurrence of immunosuppression in intestinal sepsis,has predictive value.
作者
沈晓菲
刘颂
王行舟
曹科
毛永欢
蔡大明
夏雪峰
赵阳
陆晓峰
杜峻峰
管文贤
Shen Xiaofei;Liu Song;Wang Xingzhou;Cao Ke;Mao Yonghuan;Cai Daming;Xia Xuefeng;Zhao Yang;Lu Xiaofeng;Du Junfeng;Guan Wenxian(Department of Gastrointestinal Surgery,Affiliated Drum Tower Hospital of Nanjing University Medical School,Nanjing Jiangsu Province 210008,China;Department of Intensive Care Unit,Affiliated Drum Tower Hospital of Nanjing University Medical School,Nanjing Jiangsu Province 210008,China;Chinese Acadermy of Sciences,State Key Laboratory of Menbrane Biology,Insitute of Zoology,Beijing 100101,China;Department of General Surgery,The 7th Medical Center of PLA General Hospital,Beijing 100700,China)
出处
《中华普外科手术学杂志（电子版）》
2022年第4期407-410,共4页
Chinese Journal of Operative Procedures of General Surgery(Electronic Edition)
基金
国家自然科学基金(81970500,81870393,81802846,82002082)
江苏省自然科学基金(BK20180116)。
关键词
固有淋巴细胞
腹腔内感染
质谱流式
脓毒症
Innate lymphoid cell
Intrabdominal infection
Mass spectrometry
Sepsis
