摘要
目的:初步探讨程序性死亡受体配体(programmed death-ligand 1,PD-L1)对胃癌细胞SGC7901侵袭、迁移的影响及PD-L1表达对上皮间质转化(epithelial-mesenchymal transition,EMT)的影响。方法:采用siRNA干扰技术,下调SGC7901细胞株中PD-L1的表达,采用RT-PCR法及Western blot法验证PD-L1的沉默,并将后续实验分为空白对照组、空载体组(siRNA-NC组)及PD-L1沉默组(siRNA-PD-L1组)。采用RT-PCR法及Western blot法分别对各组细胞EMT相关指标(E-cadherin、Vimentin、Snail)的mRNA和蛋白表达情况进行检测。利用MTT实验比较各组细胞的黏附能力,Transwell小室实验比较各组细胞的迁移能力及侵袭能力。结果:下调PD-L1表达后,siRNA-PD-L1组Vimentin及Snail两项指标的mRNA和蛋白表达量,细胞的黏附能力、迁移能力及侵袭能力均低于空白对照组和siRNA-NC组,E-cadherin mRNA和蛋白表达量高于其他两组,差异均具有统计学意义(P<0.001),而空白对照组和siRNA-NC组之间上述指标的差异无统计学意义。结论:PD-L1的表达影响胃癌细胞黏附、迁移、侵袭等生物学行为,促进胃癌细胞的上皮间质转化,与胃癌的预后可能存在相关性。
Objective:To explore the effect of PD-L1 on invasion and metastasis of gastric cancer cell line SGC7901 and the effect of PD-L1 expression on epithelial-mesenchymal transformation(EMT).Methods:siRNA interference technique was used to down-regulate the expression of PD-L1 in SGC7901 cell line.RT-PCR and Western blot methods were used to verify the silence of PD-L1.The subsequent experiments were divided into blank control group,empty vector group(siRNA-NC group)and PD-L1 silence group(siRNA-PD-L1 group).The mRNA and protein expression of EMT-related proteins(E-cadherin,Vimentin,Snail)in each group were detected by RT-PCR and Western blot,respectively.The adhesion ability of cells in each group was compared by MTT test,and the migration and invasion ability of cells in each group were compared by Transwell chamber test.Results:After down-regulation of PD-L1 expression,the mRNA and protein expression of Vimentin and Snail,cell adhesion,migration and invasion ability of siRNA-PD-L1 group were lower than those of blank control group and siRNA-NC group,while the expression of E-cadherin mRNA and protein were significantly higher than those of the other two groups(P<0.001),but there was no significant difference between blank control group and siRNA-NC group.Conclusion:The expression of PD-L1 affects the biological behaviors such as adhesion,migration and invasion of gastric cancer cells,and promotes the epithelial-mesenchymal transformation of gastric cancer cells,which may be related to the prognosis of gastric cancer.
作者
赵丽丽
赵文文
赵文飞
冯青青
张雪
井文君
张燕
杨风
魏红梅
ZHAO Lili;ZHAO Wenwen;ZHAO Wenfei;FENG Qingqing;ZHANG Xue;JING Wenjun;ZHANG Yan;YANG Feng;WEI Hongmei(Second Department of General Oncology,Affiliated Qingdao Central Hospital,Qingdao University,Shandong Qingdao 266042,China.)
出处
《现代肿瘤医学》
CAS
北大核心
2022年第15期2716-2722,共7页
Journal of Modern Oncology
基金
CSCO抗肿瘤免疫治疗科研基金(编号:Y-2019AZMS-0594)
山东省青岛市卫生科技计划项目(编号:2014-WZJD088)。
