摘要
为了探究拉萨河中下游纤毛虫群落的组成模式、时空多样性格局及其维持机制,本文于2015年5月和8月以及2016年10月在拉萨河中下游17个样点进行采样,采用活体观察、鲁哥氏碘液固定染色以及Wilbert蛋白银法相结合的物种鉴定方法,对纤毛虫群落结构进行了研究。通过Shannon多样性指数、Margalef丰富度指数、物种数分析群落结构时空上的差异性;通过共现网络分析纤毛虫类群之间的相互作用;通过冗余分析(redundancy analysis,RDA)探讨水体理化因子对纤毛虫群落结构的影响。结果表明,Shannon多样性指数在季节和河段间没有显著性差异;Margalef丰富度指数、物种数在河段间存在极显著性差异;中游和下游河段共现网络节点间的相关关系均以正相关为主;溶解氧(DO)、总氮(TN)、总磷(TP)、总溶解盐(TDS)是影响纤毛虫群落结构的关键因子。综上所述,拉萨河中下游纤毛虫群落结构在季节间没有显著差异,在空间上具有显著差异;纤毛虫在纲级水平上类群间的相互作用以协同作用为主导,不同类群间存在复杂的相互作用,整体上互作关系在春季较为复杂、夏季较为简单;影响拉萨河中下游纤毛虫群落结构是多个环境因子共同作用的结果。
Aims:As a high-altitude water area,the Lhasa River’s aquatic ecosystem has a high research value due to its special environmental conditions.In recent years,studies on the high-altitude water area have gradually increased,but there are few studies on the community structure of ciliates in the Lhasa River.We conducted this study to explore the composition pattern,spatial and temporal diversity pattern,and maintenance mechanism of ciliate communities in the midstream and downstream reaches of the Lhasa River.Methods:Seventeen samples were collected from the middle and lower reaches of the Lhasa River in May 2015 and August 2015,and October 2016.In-vivo observation,Rugo’s iodine solution fixation staining,and Wilbert’s protein silver method were utilized for species identification.The spatial and temporal differences of community structure were analyzed by the Shannon diversity index,Margalef index,and richness.The interactions between ciliate groups were analyzed through co-occurrence network.The effects of physical and chemical factors on ciliate community structures were investigated using redundancy analysis(RDA).Results:There was no significant difference in the Shannon index between seasons and river segments.The richness and Margalef index were significantly different among the reaches.The correlation between nodes in the midstream and downstream of the co-occurrence network is primarily positive.Dissolved oxygen(DO),total nitrogen(TN),total phosphorus(TP),and total dissolved salt(TDS)are the key factors affecting ciliate community structures.Conclusion:The ciliate community structures in the midstream and downstream of the Lhasa River exhibited no significant seasonal differences but significant spatial differences.At the class level,the interaction between ciliates was dominated by synergy,and there were complex interactions among different groups.On the whole,the interaction between ciliates was more complex in spring and simpler in summer.The community structures of ciliates in the midstream and dow
作者
杨清
张鹏
安瑞志
乔楠茜
达珍
巴桑
Qing Yang;Peng Zhang;Ruizhi An;Nanqian Qiao;Zhen Da;Sang Ba(Center for Carbon Neutrality in the Third Pole of the Earth,Tibet University,Lhasa 850000;Laboratory of Tibetan Plateau Wetland and Watershed Ecosystem,College of Science,Tibet University,Lhasa 850000)
出处
《生物多样性》
CAS
CSCD
北大核心
2022年第6期132-146,共15页
Biodiversity Science
基金
国家自然科学基金(32070418)
2022年中央财政支持地方高校改革发展专项资金(藏财预指[2022]1号)。
关键词
拉萨河
纤毛虫
群落结构
共现网络
时空分布
Lhasa River
ciliate
community structure
co-occurrence networks
spatial and temporal distribution
