摘要
采用硅胶、ODS、Sephadex LH-20和半制备HPLC等色谱技术,从粗榧中分离得到9个cephalotaxine型生物碱成分,分别为8-oxodeoxyharringtonine(1)、8-oxonordeoxyharringtonine(2)、cephafortunine A(3)、8-oxocephalotaxine(4)、deoxyharringtonine(5)、acetylcephalotaxine(6)、cephalotaxine(7)、epicephalotaxine(8)和cephalotaxinone(9)。化合物1和2为首次报道的新三尖杉酯碱类化合物,新化合物采用HR-ESI-MS、NMR、ECD等技术确定其结构。化合物1~3和5显示出显著的NF-κB转录抑制活性,其IC;分别为(3.91±0.70)、(2.99±0.45)、(7.84±0.51)、(1.46±0.17)μmol·L;。
Silica gel, octadecyl-silica(ODS), Sephadex LH-20, and semi-preparative high performance liquid chromatography(HPLC) was performed to isolate nine cephalotaxine-type alkaloids from Cephalotaxus sinensis: 8-oxodeoxyharringtonine(1), 8-oxonordeoxyharringtonine(2), cephafortunine A(3), 8-oxocephalotaxine(4), deoxyharringtonine(5), acetylcephalotaxine(6), cephalotaxine(7), epicephalotaxine(8), and cephalotaxinone(9). Compounds 1 and 2 were identified for the first time and their structures were determined by high-resolution-electrospray ionization-mass spectrometry(HR-ESI-MS), nuclear magnetic resonance(NMR), and electronic circular dichroism(ECD). Compounds 1-3 and 5 significantly inhibited the transcription of nuclear factor kappa B(NF-κB), with the half-maximal inhibitory concentration(IC;) of(3.91±0.70),(2.99±0.45),(7.84±0.51), and(1.46±0.17) μmol·L;, respectively.
作者
蔡云欣
谢晓艳
周青青
黄雄
徐金标
CAI Yun-xin;XIE Xiao-yan;ZHOU Qing-qing;HUANG Xiong;XU Jin-biao(College of Medicine,Jiaxing University,Jiaxing 314001,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2022年第11期2994-2999,共6页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(82003612)
国家级大学生创新创业训练计划项目(202110354014)。
关键词
粗榧
化学成分
三尖杉酯碱
NF-κB抑制活性
Cephalotaxus sinensis
chemical constituent
cephalotaxine-type alkaloid
NF-κB-inhibiting activity
