摘要
雷公藤种子具有休眠特性,自然播种条件下萌发周期长。该研究通过雷公藤种子的大小、形态、千粒重、生活力、含水量、理化性质及不同萌发条件下种子发芽率的测定,开发雷公藤种子快速萌发生苗的方法。研究结果表明,雷公藤种子为细柱状,外种皮厚且坚硬,种子纵长为6.69 mm,横宽为2.14 mm,厚度为1.68 mm,千粒重为8.99 g,含水量为8.86%,可溶性糖质量分数为21.3 mg·g^(-1) ,淀粉质量分数为28.9 mg·g^(-1) ,可溶性蛋白质量分数为44.2 mg·g^(-1),种子生活力为54.0%。此外,通过蒸馏水、超声、低温储藏、50℃温水、100 mg·L^(-1) 6-BA、0.6%KMnO_(4)、1%KNO_(3)、50 mg·L^(-1) NAA和100 mg·L^(-1) GA_(3)溶液处理雷公藤种子,结果表明100 mg·L^(-1) GA_(3)溶液浸泡雷公藤种子能显著促进种子的萌发。进一步用50、100、250、500、1000 mg·L^(-1) GA_(3)溶液浸泡雷公藤种子,结果表明高质量浓度(500、1000 mg·L^(-1) )的GA_(3)溶液能更高效地提高雷公藤种子的发芽率和发芽速度,萌发周期从3个月以上缩短至15 d内。研究结果对雷公藤的育种具有重要意义,为实现雷公藤种子的大规模繁殖和雷公藤种质资源的挖掘奠定重要基础。
The seeds of Tripterygium wilfordii are characterized by dormancy and a long germination cycle under natural sowing conditions.In this study,we developed a method for rapid germination of T.wilfordii seeds by analyzing the size,morphology,thousand-grain weight,viability,moisture content,physicochemical properties,and seed germination rates under different germination conditions.The seeds of T.wilfordii were fine columnar with a thick and hard outer seed coat.They had the length of 6.69 mm,the width of 2.14 mm,the thickness of 1.68 mm,the thousand-grain weight of 8.99 g,the moisture content of 8.86%,the soluble sugar content of 21.3 mg·g^(-1),the starch content of 28.9 mg·g^(-1),the soluble protein content of 44.2 mg·g^(-1),and the seed viability of only 54.0%.The seeds were respectively treated with distilled water,ultrasonication,low-temperature storage,50℃ water,100 mg·L-16-BA,0.6% KMnO_(4),1% KNO_(3),50 mg·L^(-1) NAA,and 100 mg·L^(-1) GA_(3) solution.The results showed that soaking the seeds in 100 mg·L^(-1) GA_(3) solution significantly promoted the germination.Further,the seeds were soaked in 50,100,250,500,and 1000 mg·L^(-1) GA_(3) solutions,which demonstrated that high concentration(500 mg·L^(-1),1000 mg·L^(-1))of GA_(3) solutions increased the germination rate and speed and shortened the germination cycle from more than 3 months to less than 15 days.The findings of this study are of great significance to the breeding of T.wilfordii and lay a foundation for the large-scale propagation of T.wilfordii seeds and the excavation of T.wilfordii germplasm resources.
作者
屠李婵
蔡鑫博
高杰
涂育合
刘三波
高伟
TU Li-chan;CAI Xin-bo;GAO Jie;TU Yu-he;LIU San-bo;GAO Wei(Beijing Shijitan Hospital,Capital Medical University,Beijing 100038,China;School of Traditional Chinese Medicine,Capital Medical University,Beijing 100069,China;School of Medicine,Zhejiang University City College,Hangzhou 310015,China;Yong an.State Forest Farm,Yong'an 366031,China;China Resources Sanjiu(Huangshi)Pharmaceutical Co.,Ltd.,Huangshi 435003,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2022年第11期2909-2914,共6页
China Journal of Chinese Materia Medica
基金
国家自然科学基金面上项目(81773830,81973418)。
关键词
雷公藤种子
种子性状
理化性质
休眠
发芽率
Tripterygium wilfordii seeds
seed traits
physicochemical properties
dormancy
germination rate
