摘要
目的:通过考察固本抗敏方(GKF)中各药材炮制配伍后指标成分溶出变化考察炮制对配伍的影响。方法:采用Diamosil plus C_(18)色谱柱(4.6 mm×250 mm,5μm);以乙腈(A)-0.1%甲酸水溶液(B)为流动相,梯度洗脱;流速1.0 mL/min;柱温30℃;毛蕊异黄酮苷、淫羊藿苷、朝藿定C、毛蕊异黄酮检测波长为250 nm,6-姜酚检测波长为270 nm。结果:与单味药比较,配伍后复方中朝藿定C、毛蕊异黄酮、淫羊藿苷溶出量分别增加44.54%、37.54%、165.69%,6-姜酚溶出量减少48.49%,毛蕊异黄酮苷溶出量无明显变化;与配伍用生淫羊藿复方比较,配伍用油炙淫羊藿复方中毛蕊异黄酮苷、6-姜酚、朝藿定C溶出量分别显著增加80.33%、20.93%、27.48%,毛蕊异黄酮、淫羊藿苷溶出量无明显变化。结论:配伍是GKF增效的方式,油炙淫羊藿更有助于处方药效的发挥。
Objective:The effect of processing on the compatibility of Guben Kangmin Formula(GKF)was investigated by analyzing the dissolution of index components.Methods:The separation was performed on Diamosil plus C_(18) chromatographic column(4.6 mm×250 mm,5μm)for gradient elution with acetonitrile(A)-0.1%formic acid aqueous solution(B),flow rate 1.0 mL/min,column temperature 30℃,the detection wavelength was 250 nm for calycosin-7-glucoside,calycosin,epimedin C,icariin,and 270 nm for 6-gingerol.Results:Compared with the single-herb,the dissolution of epimedin C,calycosin,and icariin increased significantly by 44.54%,37.54%,165.69%,and 6-gingerol decreased by 48.49%in the compound,no significant changes in calycosin-7-glucoside dissolution;compared with the compound with raw epimedium,the dissolution of calycosin-7-glucoside,6-gingerol,epimedin C,increased significantly by 80.33%,20.93%,27.48%,in compound with oilprocessing epimedium,no significant changes in icariin dissolution and calycosin.Conclusion:Compatibility is a synergistic method of GKF,epimedium processed by sheep fat oil is more conducive to the effectiveness of prescriptions.
作者
任原昕
赵瑞芝
王阳
REN Yuan-xin;ZHAO Rui-zhi;WANG Yang(Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China;The Second Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510120,China)
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2022年第6期3429-3433,共5页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
广东省中医药局中医药行业科研专项(No.20203005)。
关键词
淫羊藿
固本抗敏方
配伍
炮制
溶出
活性成分
Epimedium
Guben Kangmin Formula
Compatibility
Processing
Dissolution
Active components
