摘要
甲氨基阿维菌素苯甲酸盐(甲维盐)和虱螨脲是目前生产上防治草地贪夜蛾的主要杀虫剂,为评估其抗性风险,以福建省草地贪夜蛾田间种群为研究对象,在实验室抗性汰选品系选育基础上,采用数量遗传学域性状分析法并结合交互抗性测定,进行草地贪夜蛾对上述两种杀虫剂的抗性风险评估。结果表明:非连续汰选11代和10代后,草地贪夜蛾对甲维盐(F_(11))和虱螨脲(F_(10))的抗性倍数分别达30.57倍和11.35倍;抗性现实遗传力分别为0.403和0.555,且前半段筛选的抗性遗传力都远大于后半段;药剂在室内对草地贪夜蛾致死率为50%~90%时,对甲维盐和虱螨脲抗性倍数上升10倍需要汰选8~15代和6~12代。交互抗性测定显示,甲维盐汰选品系对氯虫苯甲酰胺、虱螨脲、虫螨腈无交互抗性,对茚虫威和乙基多杀菌素存在一定交互抗性。虱螨脲汰选品系对氯虫苯甲酰胺、甲维盐、茚虫威、虫螨腈和乙基多杀菌素均无交互抗性。结果表明:草地贪夜蛾对甲维盐和虱螨脲存在快速产生抗性的风险,但可通过与无交互抗性药剂轮用来延缓抗性发展。
Emamectin benzoate and lufenuron are main insecticides for controlling Spodoptera frugiperda.In order to evaluate the resistance risk of S.frugiperda to the two insecticides,field population in Fujian province was screened for resistant strains in the laboratory.Quantitative genetics domain trait analysis method and cross-resistance determination were performed on the resistant strains.The results showed that after 11 and 10 generations of non-sequential selection,the resistance levels of S.frugiperda to emamectin benzoate(F_(11))and lufenuron(F_(10))were 30.57 and 11.35 times,respectively.The realized heritability of resistance was 0.403 and 0.555,respectively,and the resistance heritability of the first half of the screening was much greater than that of the second half.When the indoor killing rate was 50%-90%,to obtain 10-fold increase of resistance,8-15 generations and 6-12 generations were needed to be selected for the two strains,respectively.The cross-resistance assays showed that the selected emamectin benzoate-resistant strain had no cross-resistance to chlorantraniliprole,lufenuron and chlorfenapyr,but had certain cross-resistance to indoxacarb and spinetoram.The selected lufenuron-resistant strain had no cross-resistance to chlorantraniliprole,emamectin benzoate,indoxacarb,chlorfenapyr and spinetoram.Therefore,S.frugiperda has the potential to evolve and develop rapid resistance to emamectin benzoate and lufenuron,but resistance development could be delayed by rotating with insecticides with no cross resistance.
作者
赵金凤
邱良妙
丁雪玲
姚凤銮
卢学松
郑宇
翁启勇
ZHAO Jinfeng;QIU Liangmiao;DING Xueling;YAO Fengluan;LU Xuesong;ZHENG Yu;WENG Qiyong(State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops,Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests,Fujian Engineering Research Center for Green Pest Management,Institute of Plant Protection,Fujian Academy of Agricultural Sciences,Fuzhou 350012,China;Fujian Agriculture and Forestry University,Fuzhou 350002,China)
出处
《植物保护》
CAS
CSCD
北大核心
2022年第4期88-93,99,共7页
Plant Protection
基金
福建省科技厅引导性项目(2020N0026)
福建省公益类科研院所基本科研专项(2020R1024008,2020R1024007)
“5511”协同创新工程(XTCXGC2021011,XTCXGC2021017)
福建省农业科学院植物保护创新团队(CXTD2021027)。
