摘要
目的探讨肿瘤坏死因子α(TNF-α)与慢性血栓栓塞性肺动脉高压(CTEPH)大鼠肺血管重塑的关系。方法2020年1-6月选取哈尔滨医科大学实验动物中心提供的Wister大鼠104只,采用随机数字表法分为CTEPH组(52只)、假手术组(52只)。CTEPH组经左颈外静脉注入栓子,制备CTEPH模型,假手术组注入等量0.9%氯化钠注射液。栓塞7 d、1个月时检测相关指标。结果栓塞7 d、1个月,假手术组血浆TNF-α[(45.62±1.65)ng/L、(46.24±2.82)ng/L]均低于CTEPH组的(47.15±1.58)ng/L、(48.85±2.96)ng/L(t=3.48、3.31,均P<0.05);栓塞后7 d、1个月,假手术组肺动脉平均压(mPAP)均低于CTEPH组(t=1.89、3.11,均P<0.05);栓塞后7 d、1个月,假手术组肺动脉TNF-αmRNA均低于CTEPH组(t=3.06、3.36,均P<0.05);栓塞后7 d、1个月,假手术组肺动脉管壁面积/管总面积(WA/TA)均低于CTEPH组(t=1.73、4.17,均P<0.05);血浆TNF-α与肺动脉TNF-αmRNA表达呈正相关(r=0.82,P<0.05);血浆TNF-α、肺动脉TNF-αmRNA与mPAP均呈正相关(r=0.62、0.73,均P<0.05),与WA/TA均呈正相关(r=0.61、0.63,均P<0.05)。结论TNF-α可能经促进肺动脉压提升、血管重塑参与CTEPH发病。
Objective To investigate the relationship between tumor necrosis factorα(TNF-α)and pulmonary vascular remodeling in rats with chronic thromboembolic pulmonary hypertension(CTEPH).Methods A total of 104 Wister rats were provided by Laboratory Animal Center,Harbin Medical University between January 2020 and June 2020 and included in this study.They were randomly divided into CTEPH group(n=52)and sham-operation group(n=52).Rats in the CTEPH group were injected with an embolus via the left external jugular vein to establish rat models of CTEPH.Rats in the sham-operation group were injected with the same amount of 0.9%sodium chloride injection.Relevant indicators were measured after 7 days and 1 month of embolization.Results After embolization for 7 days and 1 month,plasma TNF-αlevel in the sham-operation group was(45.62±1.65)ng/L and(46.24±2.82)ng/L,respectively,which were significantly lower than those in the CTEPH group[(47.15±1.58)ng/L,(48.85±2.96)ng/L,t=3.48,3.31,both P<0.05].Mean pulmonary artery pressure in the sham-operation group was significantly lower than that in the CTEPH group(t=1.89,3.11,both P<0.05).TNF-αmRNA expression in the sham-operation group was significantly lower than that in the CTEPH group(t=3.06,3.36,both P<0.05).The area/total area of pulmonary artery wall in the sham-operation group was significantly lower than that in the CTEPH group(t=1.73,4.17,both P<0.05).Plasma TNF-αlevel was positively correlated with pulmonary artery TNF-αmRNA expression(r=0.82,P<0.05).Plasma TNF-αlevel and pulmonary artery TNF-αmRNA expression were positively correlated with mean pulmonary artery pressure(r=0.62,0.73,both P<0.05)and area/total area of pulmonary artery wall(r=0.61,0.63,both P<0.05).Conclusion TNF-αmay be involved in the pathogenesis of CTEPH by increasing pulmonary artery pressure and vascular remodeling.
作者
王刚
刘登昌
颜丽莎
赵鹏
Gang Wang;Dengchang Liu;Lisha Yan;Peng Zhao(Second Department of Internal Medicine,Dezhou People's Hospital,Dezhou 253045,Shandong Province,China)
出处
《中国基层医药》
CAS
2022年第6期848-851,共4页
Chinese Journal of Primary Medicine and Pharmacy
基金
山东省医药卫生科技发展计划项目(2017WS072)。
关键词
动物
大鼠
肿瘤坏死因子Α
高血压
肺性
血管重塑
Animals
Rats
Tumor necrosis factor-alpha
Hypertension,pulmonary
Vascular remodeling
