摘要
在前期转录组数据的基础上对梅花(Prunus mume)垂枝候选基因PmTAC1(TILLER ANGLE CONTROL 1)进行了克隆及相关分析。PmTAC1编码区全长915 bp,编码304个氨基酸,且在梅花垂枝和直枝品种中无序列差异。氨基酸序列比对进一步证实PmTAC1具备IGT家族典型结构域,进化树分析表明PmTAC1与桃(Prunus persica)的PpeTAC1亲缘关系最近,亚细胞定位预测该蛋白定位于细胞核和叶绿体,蛋白疏水性分析显示其具有亲水性。PmTAC1在茎中表达最高,叶和顶芽次之。PmTAC1在垂枝品种的1年生枝条中的表达显著高于直枝品种,且在两种枝型枝条近远轴侧均有差异。垂枝品种PmTAC1的启动子序列长度为1608 bp,直枝品种启动子序列长度为1379 bp。启动子顺式元件预测到光响应、赤霉素合成、脱落酸响应顺式元件的数量在两种枝型品种间具有差异。推测PmTAC1在垂枝和直枝梅花品种中的启动子序列及表达量差异可能与垂枝性状形成有关。
A candidate gene PmTAC1(TILLER ANGLE CONTROL 1)of the weeping trait of Prunus mume was cloned and analyzed based on our previous transcriptome data.The coding sequence of PmTAC1 was 915 bp,encoding 304 amino acids,and no differences was observed between upright and weeping cultivars of mei.The amino acid sequence alignment further confirmed that PmTAC1 had the typical domain of the IGT family,and evolutionary analysis showed that the genetic relationship between PmTAC1 and PpeTAC1 was the closest.Prediction of subcellular localization and hydrophobic analysis of protein showed that the protein was located in the nucleus and chloroplast,and was hydrophilic.The expression of PmTAC1 in branches of weeping cultivars was significantly higher than that in 1-year-old branches of upright cultivars,and there were differences in the adaxial and abaxial of the two types of branches.The promoter sequences of PmTAC1 in weeping and upright mei were 1608 bp and 1379 bp respectively,which were consistent among different cultivars of the same branch types of mei.Differences were detected in the number of light responses,gibberellin,and abscisic acid response cis-elements between the two promoter sequences.In conclusion,the promoter sequence and expression level of PmTAC1 in weeping and upright cultivars of mei may be related to the formation of weeping traits.
作者
刘瑶瑶
吴严严
石岩
毛天宇
包满珠
张俊卫
张杰
LIU Yaoyao;WU Yanyan;Shi Yan;MAO Tianyu;BAO Manzhu;ZHANG Junwei;ZHANG Jie(Key Laboratory of Horticultural Plant Biology of Ministry of Education,College of Horticulture and Forestry Science,Huazhong Agricultural University,Wuhan 430070,China)
出处
《园艺学报》
CAS
CSCD
北大核心
2022年第6期1327-1338,共12页
Acta Horticulturae Sinica
基金
国家自然科学基金项目(31700619)
中央高校基本科研业务费专项基金项目(2662020YLPY006)。
