摘要
目的了解贵州省乙型流感病毒BY系和BV系毒株HA和NA基因分子进化特征,为流感的科学防控提供依据。方法统计贵州省2017—2021年流感各型别阳性比例,提取乙型流感病毒核酸,RT-PCR扩增HA和NA基因,进行序列测定14株,并从GISAID获取83株序列,对合计97株乙型流感病毒的序列进行同源性、遗传进化和氨基酸位点变异分析。结果贵州省存在甲型、BY系和BV系流感病毒的流行,BV系为优势流行型别;BY系流感病毒HA和NA基因与参考疫苗株B/PHUKET/3073/2013相比同源性分别为98.7%~99.4%和98.4%~99.6%,BV系毒株与参考疫苗株B/Colorado/06/2017相比分别为98.3%~99.3%和98.9%~99.6%;贵州省BY系毒株主要属于Y3遗传群,HA基因处于Y3-H1~2两个分支,NA基因处于Y3-N1~3三个分支,发现3株Y3系内重配株;BV系毒株主要属于V1A-2遗传群,HA基因处于V1A-2 H1~4四个分支,NA基因处于V1A-2 N1~5五个分支,发现20株V1A-2系内重配株,未发现系间重配株;关键氨基酸位点变异分析显示抗原决定簇区域位点Y3遗传群无突变,V1A-2遗传群4个主要的抗原决定簇均存在点突变且190螺旋存在移码突变,耐药位点均未发生突变。结论贵州省流感流行型别多样,乙型流感病毒与疫苗株同源性差异在增大,HA和NA基因已进化出不同分支,基因序列存在多种形式的突变,存在系内重配株和新的变种,应持续加强乙型流感病毒的监测研究。
Objective To analyze the molecular evolution characteristics of HA and NA genes of influenza B/Yamagata(BY)and influenza B/Victoria(BV)lineage viruses in Guizhou Province,aiming to provide reference for scientific prevention and control of influenza.Methods The prevalence of various types of influenza viruses in Guizhou Province from 2017 to 2021 was analyzed.The nucleic acid of influenza B viruses was extracted,and then the HA and NA genes were amplified by RT-PCR.Fourteen strains were sequenced and the sequences of 83 strains were obtained from GISAID.Homologies between the 97 influenza B viruses as well as the phylogenetic characteristics and amino acid site variations were analyzed.Results Influenza A,BY and BV lineage viruses co-circulated in Guizhou Province and BV lineage was the predominant type.The homologies of HA and NA genes were 98.7%-99.4%and 98.4%-99.6%between BY lineage viruses and the reference vaccine strain B/PHUKET/3073/2013.BV lineage viruses shared 98.3%-99.3%and 98.9%-99.6%homologies with the reference vaccine strain B/Colorado/06/2017.The BY lineage strains in Guizhou Province mainly belonged to Y3 genetic group with HA gene in two branches of Y3-H1-2 and NA gene in three branches of Y3-N1-3.Three reassortant strains were found in Y3 clade.The isolated BV lineage strains mainly belonged to V1A-2 genetic group with HA gene in four branches of V1A-2 H1-4 and NA gene in five branches of V1A-2 N1-5.Twenty reassortant strains were found in V1A-2 clade and no inter-lineage reassortants were found.Analysis of variations at key amino acid sites showed that there was no mutation at epitopes in Y3 genetic group.However,there were point mutations at four main epitopes and a shift mutation in 190 helix in V1A-2 genetic group.There was no mutation in drug resistance sites.Conclusions Various types of influenza viruses circulated in Guizhou Province.The homology between influenza B viruses and vaccine strains was decreasing.Different branches of HA and NA genes had been evolved and various forms of muta
作者
万永虎
郑勤妮
庄丽
任丽娟
蒋维佳
雷明玉
李世军
牟鸿江
郭华
Wan Yonghu;Zheng Qinni;Zhuang Li;Ren Lijuan;Jiang Weijia;Lei Mingyu;Li Shijun;Mou Hongjiang;Guo Hua(Laboratory of Viral Diseases,Guizhou Center for Disease Control and Prevention,Guiyang 550004,China)
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2022年第6期464-471,共8页
Chinese Journal of Microbiology and Immunology
基金
贵州省科技计划基金项目(黔科合基础[2020]1Y360)
贵州省卫生健康委科学技术基金项目(gzwjkj2019-2-002)
贵州省菌(毒)种和生物样本保藏研究及其资源应用(黔科合支撑[2020]4Y216)。
关键词
乙型流感病毒
HA和NA基因
遗传进化
分子特征
Influenza B virus
HA and NA genes
Genetic evolution
Molecular characteristics
