摘要
本研究探讨了二氢杨梅素对黄曲霉的抗真菌活性和潜在的抗真菌机制。首先,通过抑菌实验证明,二氢杨梅素对黄曲霉孢子和菌丝的最小抑菌质量浓度(minimum inhibitory concentration,MIC)均为4 mg/mL。通过荧光增白剂(calcofluor white,CW)和碘化丙锭(propidium iodide,PI)染色实验证明,二氢杨梅素处理后黄曲霉细胞壁和细胞膜受损。与对照组相比,1/2 MIC和MIC组黄曲霉细胞内容物释放量(OD_(260 nm))分别增加了3.14倍和5.93倍,细胞外相对电导率和pH值均升高,MIC的二氢杨梅素对黄曲霉的呼吸抑制率达25.82%。这些结果表明,二氢杨梅素通过破坏细胞壁和细胞膜的完整性以及干扰呼吸代谢发挥抗菌活性。此外,二氢杨梅素在MIC时能够完全抑制黄曲霉在花生和玉米籽粒上的萌发,因此,二氢杨梅素可作为一种有效的抗真菌天然化合物应用于粮食及农产品储藏中。
This study explored the antifungal activity and potential mechanism of action of dihydromyricetin(DMY)against Aspergillus flavus.The minimum inhibitory concentration(MIC)of DMY against the spore germination and mycelial growth of A.flavus was 4 mg/mL.Calcofluor white(CW)and propidium iodide(PI)staining proved that DMY treatment destroyed the cell wall and membrane of A.flavus.Compared with the control group,the release of A.flavus cell content(expressed as OD_(260 nm))in the 1/2 MIC and MIC groups increased by 4.14 and 6.93 times,respectively,and both the extracellular pH and relative conductivity increased.The inhibition rate of A.flavus respiration by DMY at MIC was as high as 25.82%.These results indicated that DMY exerted antifungal activity against A.flavus via disrupting the integrities of the cell wall and membrane and interfering with respiratory metabolism.In addition,DMY could effectively inhibit the germination of A.flavus spores on peanuts and corn kernels.Therefore,DMY can be used as an effective natural antifungal compound in the preservation of grains and agricultural products.
作者
李倩
赵颖
乔苏瑞
谢岩黎
LI Qian;ZHAO Ying;QIAO Surui;XIE Yanli(Henan Key Laboratory of Cereal and Oil Food Safety Inspection and Control,College of Food Science and Engineering,Henan University of Technology,Zhengzhou 450001,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2022年第13期8-14,共7页
Food Science
基金
河南工业大学高层次人才基金项目(2019BS019)
河南省教育厅自然科学研究项目(21A550005)
河南工业大学创新基金支持计划专项资助(2020ZKCJ17)
郑州市重大科技创新专项(2020CXZX0077)。
关键词
二氢杨梅素
黄曲霉
抗真菌机制
花生
玉米
dihydromyricetin
Aspergillus flavus
antifungal mechanism
peanuts
corn
