摘要
为建立高效液相色谱法同时测定忍冬藤配方颗粒中绿原酸、咖啡酸、马钱苷含量的方法,本文采用Agilent ZORBAX SB-C_(18)色谱柱,乙腈(A)—0.4%磷酸(B)为流动相,进行测定。结果显示,绿原酸进样量在0.0080~0.0801μg(r=0.9999)、咖啡酸进样量在0.0050~0.0437μg(r=0.9995)、马钱苷进样量在0.0893~0.8225μg(r=0.9999)范围内线性关系良好;平均回收率为97.9%、97.1%、98.0%,RSD均在1.0%以内。由此可见,该方法分离度高、灵敏度高、重复性较好,可用于忍冬藤配方颗粒中3种成分的含量测定。
In order to establish an HPLC method for simultaneous determination of chlorogenic acid,caffeic acid and loganin in Lonicera japonica dispensing granules,Agilent ZORBAX SB-C18 chromatographic column and Acetonitrile(a)-0.4%Phosphoric Acid(b)as mobile phase were used for determination.The results showed that the injection amount of chlorogenic acid was 0.0080-0.0801μg(r=0.9999),caffeic acid injection amount is 0.0050-0.0437μg(r=0.9995),the injection amount of loganin is 0.0893-0.8225μg(r=0.9999).The linear relationship within the range of G(r=0.9999)is good;The average recoveries were 97.9%,97.1%and 98.0%,and the RSD was within 1.0%.It can be seen that the method has high resolution,high sensitivity and good repeatability,and can be used for the determination of three components in Lonicera japonica dispensing granules.
作者
李志娇
巩长芹
李学红
张晓博
LI Zhijiao;GONG Changqin;LI Xuehong;ZHANG Xiaobo(Linyi Inspection and Testing Center,Linyi Shandong,276001,China;Qilu Pharmaceutical Co.,Ltd.)
出处
《质量安全与检验检测》
2022年第3期46-48,共3页
QUALITY SAFETY INSPECTION AND TESTING
