摘要
【目的】研究蒿柳(Salix viminalis)雌雄株幼苗在生长季不同阶段的生长与防御差异,为雌雄株差异研究及其在生产实践中的选择性应用提供指导。【方法】以蒿柳1年生雌雄扦插苗为研究对象,在大田中采用完全随机区组设计,于6-10月(6月5日、7月3日、8月4日、9月7日和10月5日)对雌雄株幼苗的生长、光合作用参数、光合色素含量、化学防御物质含量以及防御酶活性等测定后进行动态分析,比较二者差异。【结果】蒿柳雌雄幼苗株高在6月、地径在6月和7月无显著差异,其他时间株高和地径均为雄株显著高于雌株。6月雌雄株间的净光合速率(P_(n))和蒸腾速率(T_(r))无显著差异,7-10月均表现为雄株显著高于雌株;6-9月雌雄株间气孔导度(G_(s))无显著差异,仅在10月雄株显著高于雌株;6-10月整个生长季雌雄株间胞间CO_(2)浓度(C_(i))差异均不显著。雌雄株间叶绿素a(Chl a)、叶绿素b(Chl b)和类胡萝卜素(Car)含量在6月差异均不显著,7-10月各光合色素含量均为雄株显著高于雌株。雌雄株间多酚和缩合单宁含量在生长季均无显著差异,但雄株的SOD(6月除外)、POD、PPO和PAL(6月除外)活性均显著高于雌株。【结论】在有胁迫因素存在的自然环境中,蒿柳雌雄株幼苗防御物质含量无显著差异,但雌株防御酶活性较低、生长相对缓慢,可能在繁殖与生长、防御间存在进化的权衡。蒿柳雄株生长相对较快,且可解决飞絮问题,在实际应用中可优先选用。
【Objective】This study investigated sexual differences in growth and defense of Salix viminalis seedlings in different growing stages to provide guidance for gender difference research and selective application of male and female plants in practice.【Method】Male and female one-year-old cutting seedlings of S.viminalis were selected,and the growth,photosynthetic parameters,photosynthetic pigment contents,chemical defense substance content and defense enzyme activities were measured and analyzed with the design of completely randomized blocks under field experiment conditions on June 5,July 3,August 4,September 7 and October 5.【Result】There were no significant gender differences in plant height in June and ground diameter in June and July,but the height and ground diameter of male plants were significantly higher than those of female plants at other dates.The net photosynthetic rate(P_(n))and transpiration rate(T_(r))showed no significant gender difference in June,while the P_(n) and T_(r)of male plants were significantly higher than those of female plants from July to October.The stomatal conductance(G_(s))showed no significant gender difference from June to September,while the G_(s) of male plants was significantly higher than that of female plants in October.The intercellular CO_(2) concentration(C_(i))showed no significant gender difference from June to October.The contents of chlorophyll a(Chl a),chlorophyll b(Chl b)and carotenoid(Car)showed no significant gender difference in June,while the photosynthetic pigment contents of male plants were significantly higher than those of female plants from July to October.There was no significant difference in contents of secondary metabolites(polyphenols and tannins)between males and females from June to October,but the activities of SOD(except for June),POD,PPO and PAL(except for June)in male were significantly higher than those in female.【Conclusion】In the natural environment with stress factors,there was no significant differences in secondary metabolit
作者
翟飞飞
毛金梅
李海栋
张运兴
李振坚
刘俊祥
李霞
孙振元
ZHAI Feifei;MAO Jinmei;LI Haidong;ZHANG Yunxing;LI Zhenjian;LIU Junxiang;LI Xia;SUN Zhenyuan(School of Architectural and Artistic Design,Henan Polytechnic University,Jiaozuo,Henan 454000,China;Research Institute of Forestry,Chinese Academy of Forestry,Beijing 100091,China;Research Institute of Economic Forest,Xinjiang Academy of Forestry,Urumqi,Xinjiang 830063,China;College of Agriculture and Bioengineering,Heze University,Heze,Shandong 274000,China)
出处
《西北农林科技大学学报（自然科学版）》
CSCD
北大核心
2022年第8期46-54,共9页
Journal of Northwest A&F University(Natural Science Edition)
基金
河南理工大学青年骨干教师资助计划项目(2020XQG-19)
国家自然科学基金项目(31700533)
中央公益性科研机构基础研究基金项目(CAFYBB2018ZB002)
山东省自然科学基金重点项目(ZR2020KC020)。
关键词
蒿柳
雌雄异株
性别差异
化学防御
Salix viminalis
dioecy
gender difference
chemical defense
