摘要
为探究水氮耦合对沙培黄瓜叶片抗氧化酶(SOD、POD、CAT)活性、丙二醛(MDA)含量、脯氨酸(Pro)含量、产量、水分利用效率及氮肥偏生产力的影响,以期为温室沙培黄瓜科学水氮运筹提供基础理论依据,试验以“优胜美”水果黄瓜为试材,设置施氮量和灌水水平两因素并采用二次饱和D-最优设计,研究沙培黄瓜水氮耦合下叶片抗氧化酶和水氮利用的响应特征。结果表明:沙培黄瓜叶片SOD、CAT活性和Pro含量在整个生育期内呈单峰曲线变化,POD活性和MDA含量在整个生育期内呈现出上升趋势,中水中氮的处理T4和中水高氮的处理T5在全生育期内均能获得较高的抗氧化酶活性,低水高氮的处理T3在各时期内能获得最高的叶片Pro含量,灌水水平和施氮量或高或低均会造成MDA含量大量积累,降低SOD活性,在适宜的灌水水平下保证充足的氮肥供应能够得到较高的叶片POD活性和CAT活性;叶片SOD和POD活性在末瓜期时需要增施氮肥才能保持在较高水平;灌水水平决定了灌水量的多少,处理T5的产量最高为107.43 kg/hm^(2),其次是处理T4,黄瓜产量随着灌水水平和施氮量的增加均呈现出开口向下的变化趋势,符合报酬递减规律,低水高氮的处理T3可获得最高的水分利用效率,在150 kg/hm^(2)的施氮量下,可获得最高的氮肥偏生产力。综上,在T4处理(即灌水水平为80.20%,施氮量为623 kg/hm^(2))作为最优水氮运筹方案的基础上,进入末瓜期后适当增施氮肥,能够提升叶片生理代谢能力,达到高产的目标同时提升水氮利用能力。
In order to explore the effects of water-nitrogen coupling on the activities of antioxidant enzymes(SOD,POD,CAT),malondialdehyde(MDA)content,proline(Pro)content,yield,water use efficiency and nitrogen fertilizer partial productivity in sand-peened cucumber leaves to provide a basic theoretical basis for scientific water and nitrogen management of greenhouse sand culture cucumbers,in this experiment,the response characteristics of antioxidant enzymes and water and nitrogen utilization in cucumber leaves under water and nitrogen coupling were studied by setting two factors of nitrogen application rate and irrigation level and adopting the quadratic saturated Doptimal design.The results showed that:the activities of SOD and CAT and the contents of Pro in cucumber leaves showed a single peak curve during the whole growth period,while the activities of POD and MDA showed an increasing trend during the whole growth period.The higher antioxidant enzyme activities were obtained in treatment T4 of medium water nitrogen and T5 of medium water and high nitrogen.Low water and high nitrogen treatment T3 could obtain the highest Pro content in leaves in each period.High or low irrigation level and nitrogen application rate would lead to the accumulation of MDA content and reduce SOD activity.Under appropriate irrigation level,sufficient nitrogen supply could achieve higher POD activity and CAT activity in leaves.The activities of SOD and POD in leaves should be kept at a high level by increasing nitrogen application at the end of watermelon stage.The highest yield of T5 was 107.43 kg/hm^(2),followed by T4.Cucumber yield showed a downward trend with the increase of irrigation level and nitrogen application rate,which was in line with the law of decreasing returns.Low water and high nitrogen treatment T3 could obtain the highest water use efficiency.The highest partial nitrogen productivity was obtained at the nitrogen application rate of 150 kg/hm^(2).In conclusion,on the basis of T4 treatment(i.e.,80.20%irrigation level and 62
作者
马新超
轩正英
闵昊哲
齐志文
成宏宇
谭占明
王旭峰
MA Xin-chao;XUAN Zheng-ying;MIN Hao-zhe;QI Zhi-wen;CHENG Hong-yu;TAN Zhan-ming;WANG Xu-feng(College of Horticulture and Forestry,Tarim University,Aral 843300,Xinjiang,China;National and Local Joint Engineering Laboratory for Efficient and High Quality Cultivation and Deep Processing Technology of Characteristic Fruit Trees in Southern Xinjiang,Tarim University,Aral 843300,Xinjiang,China;School of Mechanical Electrification Engineering,Tarim University,Aral 843300,Xinjiang,China)
出处
《节水灌溉》
北大核心
2022年第7期58-64,共7页
Water Saving Irrigation
基金
南疆重点产业支撑计划项目(2019DB001)
新疆生产建设兵团科技攻关项目(2018DB003)
塔里木大学研究生创新训练项目(TDGRI202023)。
关键词
水氮耦合
黄瓜
叶片
抗氧化酶
产量
水分利用效率
氮肥偏生产力
water-nitrogen coupling
cucumber
leaves
enzyme activity
yield
water use efficiency
partial productivity of nitrogen fertilizer
