摘要
目的对不同w_(Ca)/w_(P)的宽带激光熔覆生物陶瓷涂层性能进行研究,探究w_(Ca)/w_(P)对涂层硬度、生物相容性及生物活性的影响规律,寻求最优试验参数,为以后制备性能优良的涂层提供参考。方法运用梯度设计思想,采用宽带激光熔覆技术,在钛合金表面分别制备w_(Ca)/w_(P)为1.35、1.40、1.45的梯度生物陶瓷涂层。利用X射线衍射仪(XRD)、扫描电镜(SEM)、金相显微镜、显微硬度仪、模拟体液浸泡试验、体外细胞试验,研究不同w_(Ca)/w_(P)对生物陶瓷涂层组织结构、生物相容性以及生物活性的影响。结果XRD结果表明,涂层主要由CaTiO_(3)、HA、Ca_(2)SiO_(4)、CaO、TCP等相组成。硬度测试表明,w_(Ca)/w_(P)=1.45的涂层平均显微硬度最高,而w_(Ca)/w_(P)=1.35的涂层平均硬度最低。体外细胞试验表明,所有涂层上的MG63分布均匀,细胞形态良好,w_(Ca)/w_(P)=1.40的涂层上的OD值增长较快,共同培养至第5天时,测得的OD值远高于其他涂层,相较于第1天的OD值增加最多。SBF浸泡试验表明,3种w_(Ca)/w_(P)的陶瓷涂层都在表面覆盖了类骨磷灰石,w_(Ca)/w_(P)=1.40的涂层,经浸泡后表面覆盖了最多的类骨磷灰石。观察细胞生长形态,细胞的许多丝状伪足紧贴在涂层上,使得细胞可以粘附在涂层上并生长,呈现出正常的梭形形态,具备较完整的细胞膜,w_(Ca)/w_(P)=1.40的涂层上粘附的细胞数量更多、分布更为均匀。结论当w_(Ca)/w_(P)=1.40时,Ca-P生物陶瓷涂层不仅拥有良好的生物矿化能力,能够形成更多类骨磷灰石,而且涂层上增殖吸附的细胞数量最多,涂层表现出良好的生物相容性和生物活性。
This paper aims to study the performance of broadband laser cladding bioceramic coatings with different w_(Ca)/w_(P),explore the influence of w_(Ca)/w_(P) on coating hardness,biocompatibility and biological activity and seek the optimal experimental parameters to provide a reference for the future preparation of coatings with excellent performance.In biomedical implant materials,bioceramic coatings prepared by laser cladding technology on titanium alloy surface have attracted wide attention due to their excellent mechanical properties and osteogenic properties.At present,relevant studies only focus on the influence of La2O_(3) and SiO_(2) doping amount on the formation of HA+TCP and coating biological properties,and do not involve the influence of different w_(Ca)/w_(P) on coating properties.Therefore,this paper adopts the broadband laser cladding technology and the composition gradient design idea.The titanium alloy Ti-6Al-4V is used as the matrix material,the coating material is CaHPO_(4)·2H_(2)O,CaCO_(3) and Ti powder,and the doping materials are selected as 0.6wt.%La_(2)O_(3) and 10wt.%SiO_(2).This paper adopted to gradient design idea,using broadband laser cladding technology.Gradient bioceramic coatings with w_(Ca)/w_(P) of 1.35,1.40 and 1.45 were prepared.And by using X-ray diffractometer(XRD),scanning electron microscope(SEM),metallographic microscope,microhardness tester,simulated body fluid immersion test,in vitro cell test,system research different w_(Ca)/w_(P) of biological ceramic coating structure,the effects of different w_(Ca)/w_(P) on the coating structure,biocompatibility and bioactivity of bioceramic coatings were studied.the influence of the biocompatibility and bio activity,to seek the optimal experimental parameters,provide a reference for future preparation of high performance coating.XRD shows that the coating is mainly composed of CaTiO_(3),HA,Ca_(2)SiO_(4),CaO and TCP phases.When w_(Ca)/w_(P)=1.40,the number of HA+TCP is the largest.Hardness test shows that the higher the w_(Ca)/w_(P) i
作者
彭兰
张国芬
罗迪迪
王琴
甘英睿
朱玉发
刘其斌
PENG Lan;ZHANG Guo-fen;LUO Di-di;WANG Qin;GAN Ying-rui;ZHU Yu-fa;LIU Qi-bin(College of materials and metallurgy,Guizhou University,Guizhou Guiyang 550025,China)
出处
《表面技术》
EI
CAS
CSCD
北大核心
2022年第7期363-369,共7页
Surface Technology
