摘要
为了研究铁皮石斛菌根共生系统的Long noncoding RNA(lncRNA)调控机制,采用高通量测序方法对铁皮石斛栽培苗和组培苗lncRNA进行检测,并分析显著差异转录本。结果显示:2组4个样品共测出26543个lncRNA转录本,在实验组中显著上调的有186组,显著下调的有183组。GO富集分析共获得1275组数据,具有显著性差异的基因数最多的是细胞核;KEGG富集分析共获得20条通路,其中富集程度最显著的是苯丙素生物合成途径。选取参考基因已知的转录本,得到10组显著上调的lncRNA,10组显著下调的lncRNA,共涉及5条富集通路,分别在淀粉和蔗糖代谢途径和苯丙素生物合成途径中上调,在植物激素信号转导和内质网中的蛋白质加工途径中下调,剪接体途径中则同时存在表达上调和下调。以上结果可为进一步研究铁皮石斛菌根共生机制提供参考,对相关功能进行预测。
In order to investigate the regulatory mechanism of long noncoding RNA(lncRNA)in Dendrobium officinale mycorrhizal symbiotic system,high-throughput sequencing method was adopted to detect the lncRNA of Dendrobium officinale tissue cultured seedings and cultivated seedings,and the transcripts with significant differences were analyzed.The results showed that 26543 lncRNA transcripts were detected in 4 samples of two groups.In the experimental group,186 were significantly up-regulated and 183 were significantly down-regulated.1275 sets of data were obtained from GO enrichment analysis,of which the cell nucleus had the most genes with significant difference.20 pathways were obtained from KEGG enrichment analysis,of which the phenylpropanoid biosynthesis pathway was the most significantly enriched.10 sets of significantly up-regulated lncRNAs and 10 sets of significantly down-regulated lncRNAs were obtained from the transcripts with known reference genes,involving 5 enriched pathways,which were up-regulated in the starch and sucrose metabolic pathway and phenylpropanoid biosynthesis pathway,down-regulated in the plant hormone signal transduction pathway and protein processing pathway in endoplasmic reticulum.In the spliceosome pathway,there exist both up-regulated and down-regulated expression simultaneously.The above results can provide a reference for further study on the mycorrhizal symbiotic mechanism of Dendrobium officinale and be used to make predictions on the related functions.
作者
钱央央
黄路瑶
范浩宇
徐涛
QIAN Yangyang;HUANG Luyao;FAN Haoyu;XU Tao(College of Life Sciences and Medicine,Zhejiang Sci-Tec University,Hangzhou 310018 China)
出处
《浙江理工大学学报（自然科学版）》
2022年第4期615-622,共8页
Journal of Zhejiang Sci-Tech University(Natural Sciences)
基金
浙江省重点研发计划项目(2015C02030)。
关键词
铁皮石斛
菌根
高通量测序
lncRNA
差异性表达
Dendrobium officinale
mycorrhiza
high-throughput sequencing
lncRNA
differential expression
