摘要
老鹰茶是一种重要的民族特色类茶饮品和保健茶,在我国民间有着较长的饮用历史。用于制作老鹰茶的基源植物主要为5种樟科植物(毛豹皮樟、狭叶润楠、川黔润楠、红果黄肉楠和香叶子),其遗传学与分子生物学等方面的基础研究十分缺乏,导致老鹰茶树种质资源发掘与利用等工作支撑理论薄弱,制约了产业发展。本研究首次利用流式细胞术和K-mer频数分析2种方法对5种老鹰茶树的基因组大小及基本特征进行了分析。流式细胞术以番茄(929 Mb)和水稻(430 Mb)为对照标品;K-mer频数分析基于5个物种基因组DNA的Illumina测序数据(深度50×以上)进行。主要研究结果如下:(1)通过流式细胞术测定的毛豹皮樟基因组大小约为1150.56 Mb,狭叶润楠约为999.83 Mb,川黔润楠约为1471.99 Mb,红果黄肉楠约为1123.42 Mb,香叶子约为1350.06 Mb;(2)K-mer频数分析估测毛豹皮樟基因组大小为1025.91 Mb,杂合率为1.29%,重复率为49.98%;狭叶润楠基因组大小为1024.79 Mb,杂合率为1.41%,重复率为42.51%;川黔润楠基因组大小为1234.90 Mb,杂合率为1.23%,重复率为50.86%;红果黄肉楠基因组大小为1014.27 Mb,杂合率为1.32%,重复率为41.82%;香叶子基因组大小为1258.00 Mb,杂合率为0.74%,重复率为54.89%;(3)毛豹皮樟、狭叶润楠、川黔润楠和红果黄肉楠4种老鹰茶树属于高杂合基因组,香叶子属于微杂合基因组,而这5个物种的基因组重复度都属于中等。本研究的结果为后续的老鹰茶树单个物种基因组测序策略或泛基因组研究计划等工作提供了可参考的基础数据。
Hawk-tea is a kind of refreshing and folk tea drink with a long drinking history in China.The primary plants used for making Hawk-tea are mainly five trees from Lauraceae family,including Litsea coreana var.lanuginosa(Migo)Y.C.Yang&P.H.Huang,Machilus rehderi C.K.Allen,Machilus chuanchienensis S.K.Lee,Actinodaphne cupularis(Hemsl.)Gamble,and Lindera fragrans Oliv.Whereas their studies on genetics and molecular biology remain preliminary with less supports on the exploration and utilization of these germplasm resources,which restricts the development of the Hawk-tea industry.In this study,flow cytometry and K-mer frequency analysis were performed to estimate the genome size and genomic characteristics of the five tea-like species.In flow cytometry the genomes of tomato(929 Mb)and rice(430 Mb)were used as internal control,and K-mer frequency analysis was performed using Illumina sequencing data(depth above 50×).The main results are as follows:(1)By flow cytometry,the genome size of L.coreana var.lanuginosa(Migo)Y.C.Yang&P.H.Huang was 1150.56 Mb,M.rehderi C.K.Allen was 999.83 Mb,M.chuanchienensis S.K.Lee was 1471.99 Mb,A.cupularis(Hemsl.)Gamble was 1123.42 Mb,and L.fragrans Oliv.was 1350.06 Mb;(2)By K-mer frequency analysis,the genome size of L.coreana var.lanuginosa(Migo)Y.C.Yang&P.H.Huang was 1025.91 Mb,with a heterozygosity rate of 1.29%and repetition rate of 49.98%;the genome size of M.rehderi C.K.Allen was 1024.79 Mb,with a heterozygosity rate of 1.41%and repetition rate of 42.51%;the genome size of M.chuanchienensis S.K.Lee was 1234.90 Mb,with a heterozygosity rate of 1.23%and repetition rate of 50.86%;the genome size of A.cupularis(Hemsl.)Gamble was 1014.27 Mb,with a heterozygosity rate of 1.32%and repetition rate of 41.82%;the genome size of L.fragrans Oliv.was 1258.00 Mb,with a heterozygosity rate of 0.74%and repetition rate of 54.89%.(3)Except for L.fragrans Oliv.,four hawk-tea trees showed high heterozygous genome with medium repetitive elements.The results of this study can provide information for the ge
作者
哈登龙
石冠红
刘争
熊彪
HA Deng-long;SHI Guan-hong;LIU Zheng;XIONG Biao(Henan Jigongshan National Nature Reserve Administration,Xinyang 464099;College of Tea Science at Guizhou University,Guiyang 550025)
出处
《植物遗传资源学报》
CAS
CSCD
北大核心
2022年第4期1166-1174,共9页
Journal of Plant Genetic Resources
基金
国家自然科学基金(31900272)。
