摘要
目的探究北豆根治疗溃疡性结肠炎(Ulcerative Colitis,UC)的作用机制。方法首先以“北豆根”为关键词,检索TCMSP数据库,获取药材的已知化学成分和靶点,再以口服生物利用度和药物相似性为条件筛选得到有效成分。然后在GeneCards数据库检索UC靶点,取成分靶点与疾病靶点交集基因。将药物、成分和交集基因数据导入Cytoscape 3.2.1软件中绘制“药物-有效成分-靶点”网络,并进行分析。应用STRING数据库绘制靶点蛋白互作网络(PPI),度值高的靶点蛋白作为北豆根治疗UC的关键靶点。应用DAVID数据库进行基因功能注释分析和功能富集分析。结果筛选获得北豆根10个有效成分,78个对应靶点,UC相关基因3982个;“药物-有效成分-靶点”网络中与UC相关的靶点44个。PPI核心网络包含43个蛋白,分析结果显示:北豆根治疗UC可能与IL6、TNF、MYC、CASP3、MAPK14、PTGS2、NOS3、ESR1、RELA、MMP2等有关。基因功能注释分析和功能富集分析结果显示:北豆根治疗UC是通过参与细胞增殖与凋亡调控、信号传导、转录调控及药物反应等生物过程,调控TNF、PI3K-Akt、T细胞受体等信号通路等发挥作用。结论北豆根中多个成分作用于多个靶点和通路,发挥治疗UC的作用。
Objective To explore the mechanism of Menispermi Rhizoma in the treatment of ulcerative colitis(UC).Methods The parameters of Oral Bioavailability(OB),Drug-Like(DL)and corresponding targets were obtained by using TCSSP database,and the active components were screened.UniProt database was used to find the official gene names of the component targets of Menispermi Rhizoma,and GeneCards database was used to find the targets of UC,the intersection gene of the component target and the disease target were obtained.Cytoscape 3.2.1 was used to construct a"drug-active ingredient-target"network,construct a target protein interaction network(PPI)using the STRING database.GO enrichment analysis and KEGG pathway annotation analysis were conducted using David database.Results A total of 10 active components,78 corresponding targets,3982 UC related genes and 44 targets related to UC in drug-component-target network were screened out.The PPI network contains 43 proteins.Protein Interaction Network analysis indicated that UC might be related to IL6,TNF,MYC,CASP3,MAPK14,PTGS2,NOS3,ESR1,RELA and MMP2.In addition,GO enrichment analysis and KEGG pathway enrichment showed that Menispermi Rhizoma could treat UC through biological processes such as transcriptional regulation,cell proliferation and apoptosis regulation,signal transduction and drug response.TNF pathway,PI3K-Akt pathway,T cell receptor signal pathway play a role in the treatment of UC.Conclusion Several components of Menispermi Rhizoma act on multiple targets and pathways,and play a role in the treatment of UC.
作者
张科
宋崟
李波
谷冬梅
李正翔
袁恒杰
ZHANG Ke;SONG Yin;LI Bo;GU Dongmei;LI Zhengxiang;YUAN Hengjie(Department of Pharmacy,Tianjin Medical University General Hospital,Tianjin 300052,China;Department of Clinical Laboratory Medicine,Tianjin Medical University General Hospital,Tianjin 300052,China)
出处
《安徽医药》
CAS
2022年第8期1672-1675,I0003,共5页
Anhui Medical and Pharmaceutical Journal
关键词
结肠炎
溃疡性
蝙蝠葛属
北豆根
网络药理学
有效成分
靶点
通路
作用机制
Colitis,ulcerative
Menispermum
Menispermi Rhizoma
Network pharmacology
Active ingredient
Target
Pathway
Mechanism
