摘要
人体内常见的多胺(polyamines,PAs)主要包括腐胺(putrescine,Put)、亚精胺(spermidine,Spd)、精胺(spermine,Spm)和乙酰化多胺(acetylpolyamines,Ac-PAs)。多胺水平的失调与多种癌症的发生和发展紧密相关,因此多胺被认为是多种癌症的潜在生物标志物,同时多胺的合成、分解途径也成为癌症治疗的重要靶点。随着多种检测技术被应用于生物样品中多胺的分析,进一步验证了多胺在癌症筛查和临床诊断中的应用价值。本文简要综述了多胺检测技术及其在生物样品分析中的应用,主要包括气相色谱、气相色谱-质谱、液相色谱以及液相色谱-质谱等,此外,还简单介绍了新兴的多胺检测技术。同时,本文指出了生物样品中多胺的检测分析过程中存在的问题并给出了解决策略,并指明了多胺检测技术发展的方向,以期为多胺检测技术的研究和在癌症的早期筛查和临床诊断中的应用提供一定的参考。
The ubiquitously existed polyamines(PAs)in human body mainly include putrescine,spermidine,spermine and acetylpolyamines.Dysregulations of polyamines levels are closely related to the occurrence and development of many human cancers.Therefore,not only polyamines were prevalently regarded as potential biomarkers for a variety of cancers,but also the synthesis and catabolism pathway of polyamines was considered as the important target for cancer treatment.With lots of techniques have been applied to the determination of polyamines,the application value of polyamines in cancer screening and clinical diagnosis was furtherly confirmed.This paper concisely reviewed the detection technologies of polyamines and their applications in biological samples analysis,which mainly include gas chromatography,gas chromatographymass spectrometry,liquid chromatography and liquid chromatography-mass spectrometry,and also briefly introduced the progresses of recently emerged polyamine detection technologies.Meanwhile,this work pointed out the limitations in the detection and analysis of polyamines in biological samples,and proposed corresponding solutions that demonstrated the direction for the development of polyamine detection technologies.Finally,we hope to provide some references for the research of polyamine detection and their applications in the early screening and clinical diagnosis of cancers.
作者
石闯
刘森
SHI Chuang;LIU Sen(Key Laboratory of Fermentation Engineering of Ministry of Education&National“111”Center for Cellular Regulation and Molecular Pharmaceutics,School of Bioengineering and Food,Hubei University of Technology,Wuhan 430068,China)
出处
《生命的化学》
CAS
2022年第1期119-127,共9页
Chemistry of Life
基金
国家自然科学基金项目(31971150)
湖北省杰出青年基金项目(2019CFA069)。
关键词
多胺
多胺代谢物
多胺检测
polyamines
polyamines metabolites
polyamines detection
