摘要
基于CRISPR的单细胞转录组测序技术是将单细胞转录组测序技术与CRISPR集中筛选技术结合,用于研究传统技术无法完成的细胞异质性、细胞谱系追踪、细胞互作及空间位置、药物作用靶点预测和鉴定等。本文简单比较了六种最常用的单细胞转录组测序技术,重点介绍了五种基于CRISPR的单细胞转录组测序技术及应用研究,并对其推广应用所面临的挑战作出了分析,旨在为单细胞转录组测序技术的应用及创新提供参考。
CRISPR-based single-cell transcriptome sequencing technology is to combine single-cell RNAsequencing with CRISPR-pooled screens to study cell heterogeneity,cell lineage tracking,cell interaction and spatial location,as well as to predict and identify drug action targets that can not be completed by traditional technologies.We briefly compared the six most commonly used single-cell transcriptome sequencing technologies.Especially,we focused on five CRISPR-based single-cell transcriptome sequencing technologies and their applications and analyzed their potential challenges.We provided useful information for the applications and innovations of CRISPR-based single-cell RNA-sequencing.
作者
郭俊璠
黄行许
乔云波
GUO Junfan;HUANG Xingxu;QIAO Yunbo(School of Life Science and Technology,ShanghaiTech University,Shanghai 201210,China;School of Life Sciences,Guangzhou University,Guangzhou 510006,China)
出处
《生命的化学》
CAS
2022年第1期1-11,共11页
Chemistry of Life
