摘要
蜂蜜是传统的天然保健食品,然而却因市场鱼龙混杂而饱受诟病。如何有效区分成熟蜂蜜和商超脱水蜜等不同品质蜂蜜,对于确保蜂蜜市场的健康发展尤为重要。根据蜜蜂可分辨蜂蜜品质的仿生学原理,本文利用蜜蜂口器组织修饰玻碳电极制备组织传感器,采用循环伏安法检测蜂蜜样品。根据传感器的检测信号,构建检测样品的线性回归分析模型,确定传感器能够辨别的最佳质量浓度并进行模型和辨别效果的验证。结果显示,该组织传感器结合构建的模型能有效辨别成熟蜜、商超蜂蜜和果葡糖浆,辨别蜂蜜的最佳质量浓度区间为0.16~0.18 g/mL,辨别成熟蜜和果葡糖浆的最佳质量浓度区间为0.08~0.1 g/mL,结果稳定、可靠。结论:确定了利用蜜蜂组织制备生物传感器检测蜂蜜品质的可行性,结合蜂蜜标准品的线性模型能够稳定、可靠地辨别待测蜂蜜的种类,为蜂产品检测提供了新方法。
Honey is a traditional natural health food,but the market is mixed and criticized.It is very important for the healthy development of honey market to distinguish mature honey from commercial dehydrated honey.According to the bionics principle that bees can distinguish the quality of honey,a tissue sensor was prepared by using a glassy carbon electrode modified by honeybee mouthparts tissue,and the honey samples were detected by cyclic voltammetry.According to the detection signal of the sensor,the linear regression analysis model of the detection sample was built to determine the best mass concentration that the sensor could identify,and the model and the discrimination effect were verified.The results showed that the model combined with the tissue sensor could effectively identify mature honey and commercial dehydrated honey,and the optimal concentration range was 0.16-0.18 g/mL,the optimal concentration range of mature honey and fructose syrup is 0.08-0.1 g/mL,and the results were stable and reliable.These data proved that honeybee tissue sensor can be used for honey detection,and the linear model of honey standard products can stably and reliably identify the types of honey to be tested.
作者
张军飞
杨雪梅
孙惠
张楠
安传远
吴帆
李红亮
Zhang Junfei;Yang Xuemei;Sun Hui;Zhang Nan;An Chuanyuan;Wu Fan;Li Hongliang(Zhejiang Provincial Key Laboratory of Biometrolory and Inspection&Quarantine,College of Life Sciences,China Jiliang University,Hangzhou 310018;Xinjiang Provincial Professional Cooperatives of Beekeepers in Xinyuan County,Beitun 750300,Xinjiang)
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2022年第6期307-314,共8页
Journal of Chinese Institute Of Food Science and Technology
基金
浙江省基础公益计划项目(LGN22C170001)
国家自然科学基金项目(3217030483,32000331)
浙江省自然科学基金项目(LQ21C030007)。
关键词
蜂蜜
蜜蜂口器组织传感器
循环伏安法
峰值电流变化比
品质辨别
honey
bee mouthparts tissue sensor
cyclic voltammetry
peak current change ratio
quality distinguish
