摘要
探讨了人参(Panax ginseng C. A. Meyer)根腐病离体抗性筛选方法的可行性,初步建立了一种快速高效的人参根腐病抗性筛选的方法。采用离体叶片、根块2种方法进行接种,结果表明,2种方法接种的发病情况基本一致,且用离体叶片比用根块筛选操作更简单,观察更方便;采用菌块、菌液2种接种方法分别对人参叶片的正、反面进行接种,结果表明,用菌块正面接种发病效果更好。进一步通过人参须回接试验初步证明了离体叶片抗性筛选方法的可行性。通过对不同品系人参进行接菌,发现FS抗病能力最高,FX2抗病能力最低。
The feasibility of the screening method for root rot resistance of Panax ginseng C. A. Meyer in vitro was discussed,and a rapid and efficient screening method for root rot resistance of Panax ginseng was preliminarily established. Two methods of in vitro leaf and root block inoculation were used in the experiment. The results showed that the incidence of the two methods was basically the same,and the in vitro leaf was more simple and convenient to observe than the root block screening. In the experiment,the positive and negative sides of ginseng leaves were inoculated with two inoculation methods,i. e.,bacterial block and bacterial liquid,respectively. The results showed that the positive inoculation with bacterial block had a better incidence effect. Furthermore,the feasibility of the screening method for resistance of isolated leaves was preliminarily proved by the ginseng whisker backcross test. By inoculating different strains of ginseng,it was found that FS had the highest disease resistance and FX2 had the lowest disease resistance.
作者
张晶晶
张宁
侯微
曲正义
李亚丽
郑培和
ZHANG Jing-jing;ZHANG Ning;HOU Wei;QU Zheng-yi;LI Ya-li;ZHENG Pei-he(Institute of Special Animal and Plant Science,Chinese Academy of Agricultural Sciences,Changchun 130112,China)
出处
《湖北农业科学》
2022年第11期76-79,共4页
Hubei Agricultural Sciences
基金
国家重点研发计划课题(2017YFC1702102)
中央级公益性科研院所基本科研业务费专项(1610342019015)
中国农业科学院科技创新工程项目(CAAS-ASTIP-2016-ISAPS)。
