摘要
为了分析禽腺病毒(Fowl adenovirus,FAdV)在河南省新乡市的流行和变异情况,于2020年从新乡市周边养鸡场采集15份疑似FAdV感染的肝脏样品,通过PCR扩增获得病毒Hexon基因部分核酸序列,对获得的核酸序列进行同源性分析,并通过动物回归试验进一步评价其毒力。结果显示,15份样品中有12份扩增出约300 bp的目的条带,占采集样品的80%;进一步测序证实,分离毒株为同一毒株,命名为XX株。扩增片段经BLAST比对,分离毒株与FAdV-4型同源性大于95%,而与FAdV-1和鸭FAdV-Ⅰ同源性较低。动物回归试验结果显示,XX株对3周龄SPF鸡致病性较强,死亡率可达60%;剖检可见典型的心包积液和肝脏质脆、出血,与FAdV-4感染所致病变一致。表明本次分离的毒株具有较强的致病力。
In order to study the prevalence and variation of fowl adenovirus in Xinxiang area of Henan Province,liver samples of 15 chickens suspected of fowl adenovirus were collected from poultry farms in Xinxiang in 2020,and partial nucleic acids of Hexon gene were obtained by PCR.The obtained nucleic acid sequences were used for comparison and homology analysis,and the viral virulence was further evaluated by animal regression test.The results showed that a band of about 300 bp was amplified in 12 of the 15 samples collected,which accounted for 80%.Sequencing results showed that the obtained strains were the same strain,named as XX strain,which had more than 95%homology with FAdV-4,but low homology with FAdV-1 and duck adenovirus group I.The results of animal regression test showed that the XX strain was highly pathogenic to SPF chickens aged three weeks,and the mortality rate was up to 60%.Necropsy showed typical pericardial effusion,liver fragility,and hemorrhage,consistent with FAdV-4 infection.This indicated that the virus strain isolated in this study had strong virulence.
作者
王俊娜
李凌薇
王秋霞
师雯
张新
刘兴友
姜金庆
裴大伟
WANG Junna;LI Lingwei;WANG Qiuxia;SHI Wen;ZHANG Xin;LIU Xingyou;JIANG Jinqing;PEI Dawei(College of Animal Science and Veterinary Medicine,Henan Institute of Science and Technology,Xinxiang 453003,China;College of Life Science,Xinxiang University,Xinxiang 453003,China;Houde Food Co.Ltd.,Liaoyuan 136200,China)
出处
《河南农业科学》
北大核心
2022年第5期133-139,共7页
Journal of Henan Agricultural Sciences
基金
河南省科技攻关项目(202102110254
202102110102)
河南省高等学校重点科研项目(21A230003)
动物疫病防控与残留物免疫检测河南省高校科技创新团队支持计划项目(20IRTSTHN025)。
