摘要
【目的】分析玉米大斑病菌寄主选择性毒素(host selective toxin,HST)生物合成基因簇Cluster 397.3的结构,以及该基因簇组成基因在玉米大斑病菌侵染过程中的表达情况,为玉米大斑病菌HST的结构和功能研究奠定基础。【方法】以玉米大斑病菌23号生理小种et28a和01-23菌株,1号生理小种ny001和01-11菌株,玉米感病自交系B37为材料,利用antiSMASH技术预测玉米大斑病菌次生代谢产物合成基因簇,通过Synteny共线性分析获得玉米大斑病菌HST生物合成基因簇Cluster 397.3,并对其基因组成和结构进行分析,再利用RNA-Seq技术分析该基因簇的组成基因在玉米大斑病菌侵染玉米叶片3,5,7,10 d的表达情况。【结果】玉米大斑病菌次生代谢产物合成基因簇Cluster 397.3与交链链格孢(Alternaria alternata)ACT-毒素合成基因簇有保守的共线性,含有1个聚酮合酶(PKS)、1个氨基甲酰磷酸合成酶(CPS)、1个短链脱氢酶/还原酶(SDR)、1个锌结合氧化还原酶(ZOR)、1个保守假定蛋白(CHP)、2个细胞色素P450(P450)及2个耐药转运蛋白(DRT)等9个基因。其中PKS与ACT-毒素合成酶基因ACTTS3同源,编码的聚酮合酶包括PksD、PS-DH、Methyltransf_12、PKS_KR、PKS_NbtC superfamily和NADB_Rossmann superfamily 6个典型结构域。RNA-Seq分析结果表明,将玉米大斑病菌接种玉米叶片3,5,7,10 d后,Cluster 397.3组成基因均能表达,且以3 d时表达量整体较高;除CHP基因外,01-11菌株中基因表达量均显著高于01-23菌株。【结论】Cluster 397.3基因表达差异与玉米大斑病菌生理小种的分化和致病特异性有关,该基因簇可能参与了玉米大斑病菌寄主选择性毒素的生物合成。
【Objective】This study analyzed the structure of the host selective toxin(HST)biosynthetic gene cluster of Setosphaeria turcica and detected the expression of the cluster genes during the infection of Setosphaeria turcica to provide foundation for understanding structure and function of host selective toxin of Setosphaeria turcica.【Method】Strains et28a and 01-23 of race 23,strains ny001 and 01-11 of race 1 and susceptible inbred line B37 of maize were used to predict the gene clusters of secondary metabolites biosynthesis of Setosphaeria turcica using the antiSMASH technique.The gene Cluster 397.3 of HST biosynthesis was obtained by Synteny collinearity analysis and the composition and structure were analyzed.RNA-Seq technique was used to analyze the expression of the cluster genes on days 3,5,7 and 10 during the infection of maize leaves.【Result】Cluster 397.3 of secondary metabolites of Setosphaeria turcica had a conservative collinearity with the ACT-toxin biosynthesis gene cluster of Alternaria alternata.It contained nine coding genes including one polyketide synthase(PKS),one carbamoyl phosphate synthase(CPS),one short-chain dehydrogenase/reductase(SDR),one zinc binding dehydrogenase(ZOR),one conserved hypothetical protein(CHP),two cytochrome P450(P450)and two drug resistance transporters(DRT).PKS was homologous to ACTTS3 of ACT-toxin synthase and encoded six typical domains of PksD,PS-DH,Methyltransf_12,PKS_KR,PKS_NbtC superfamily and NADB_Rossmann superfamily.The results of RNA-Seq analysis revealed that Cluster 397.3 was expressed 3,5,7 and 10 days after inoculation of corn leaves by Setosphaeria turcica,and the expression levels on day 3 was the highest.Except CHP,the expression of all cluster genes in strain 01-11 was significantly higher than that in strain 01-23.【Conclusion】The differential expression of Cluster 397.3 was related the differentiation of physiological races and pathogenicity of Setosphaeria turcica and it may be involved in the biosynthesis of host-selective toxins of Seto
作者
张淑红
高凤菊
武秋颖
张运峰
范永山
ZHANG Shuhong;GAO Fengju;WU Qiuying;ZHANG Yunfeng;FAN Yongshan(Tangshan Key Laboratory of Agricultural Pathogenic Fungi and Toxins,Department of Life Sciences,Tangshan Normal College,Tangshan,Hebei 063000,China)
出处
《西北农林科技大学学报（自然科学版）》
CSCD
北大核心
2022年第7期84-91,共8页
Journal of Northwest A&F University(Natural Science Edition)
基金
河北省自然科学基金项目(C2019105055)
唐山市人才项目(A202007009)
唐山师范学院科研项目(2021B30,2021B34)。
关键词
玉米大斑病菌
寄主选择性毒素
基因簇
表达分析
Setosphaeria turcica
host-selective toxin
gene cluster
expression analysis
