摘要
目的:研究利多卡因对人口腔鳞癌细胞HN13增殖、凋亡的影响及机制。方法:常规条件下培养人口腔鳞癌细胞HN13,分为对照组(NC,不添加任何药物)、利多卡因低(40μmol/L)、中(400μmol/L)、高(4 000μmol/L)剂量组、阳性对照组(PC,100μmol/L顺铂),采用CCK-8法测定细胞OD值,分析不同浓度利多卡因对癌细胞增殖的影响;采用Annexin V-FITC/PI双染法结合流式细胞术检测不同浓度利多卡因对HN13细胞凋亡的影响。Western blot检测各组细胞PI3K/Akt途径中关键分子p-PI3K、p-Akt、Bcl-2等蛋白表达差异。结果:CCK-8结果显示,与对照组相比,利多卡因400μmol/L、4 000μmol/L处理组细胞存活数减少(P<0.05)。流式细胞术检测结果显示400μmol/L、4 000μmol/L利多卡因处理组细胞凋亡率提高(P<0.05)。Western blot结果显示,与NC对照组相比,利多卡因处理后各组细胞中p-PI3K、p-Akt、Bcl-2表达明显降低(P<0.05),其中4 000μmol/L处理组与顺铂处理组差异无统计学意义(P>0.05)。结论:与对照组相比,利多卡因通过抑制PI3K/Akt途径抑制HN13细胞增殖,促进细胞凋亡。
Objective:To study the effect of lidocaine on proliferation and apoptosis of human oral cancer cells HN13 and its mechanism.Methods:Human oral squamous cells HN13 were cultured under normal conditions,and grouped as control group(NC,don′t add any drugs),lidocaine low(40 μmol/L),medium(400 μmol/L),high(4 000 μmol/L)doses groups,and positive control group(PC,100 μmol/L cisplatin).OD value of cells was evaluated by CCK-8 method to analyze effects of different concentrations of lidocaine on cancer cell proliferation;Annexin V-FITC/PI double staining combined with flow cytometry was used to detect effects of different concentrations of lidocaine on apoptosis of cancer cells.Western blot was used to detect differences in expressions of p-PI3K,p-Akt,Bcl-2 in PI3K/Akt pathway in each group.Results:CCK-8 assay showed that compared with control group,number of cells in groups treated with 400 μmol/L and 4 000 μmol/L lidocaine were reduced(P0.05).Conclusion:Compared with control group,lidocaine inhibits cell HN13 proliferation and promotes cell apoptosis by inhibiting PI3K/Akt pathway.
作者
常冰梅
肖瑞琳
赵虹
杨小庆
张栋
王丽丽
CHANG Bingmei;XIAO Ruilin;ZHAO Hong;YANG Xiaoqing;ZHANG Dong;WANG Lili(Department of Biochemistry and Molecular Biology,Shanxi Medical University,Taiyuan 030001,China)
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2022年第10期1218-1221,共4页
Chinese Journal of Immunology
基金
山西省应用基础研究项目(201801D121310)
山西省重点研发计划项目(201903D321101)。
