摘要
以甘肃煤层气井排采水和内蒙古地区煤层缝隙渗水作为本源菌,活性污泥作为外源菌,经驯化后分别对两地区煤在190 L大型发酵罐内进行生物降解产甲烷实验。分析了两种菌源降解煤过程中发酵罐压力、甲烷体积分数、产甲烷总量、产气规律及两种菌源降解本地区煤产甲烷的代谢途径。结果表明:经驯化后的甘肃地区本源菌在对本地区煤生物降解产气过程中,发酵罐最高压力在第32天达45.80 kPa,甲烷体积分数为30.57%,产甲烷总量为8.97 L,加入营养液后,发酵罐最高压力在第15天达121.00 kPa,甲烷体积分数为38.22%,产甲烷总量为29.55 L,产甲烷率为2.95 mL/g;经驯化后的内蒙古地区本源菌对本地区煤生物降解产气过程中,发酵罐最高压力在第21天达70.56 kPa,甲烷体积分数为32.78%,产甲烷总量为14.89 L,加入营养液后,发酵罐最高压力在第14天达140.73 kPa,甲烷体积分数为40.02%,产甲烷总量为36.10 L,产甲烷率为3.61 mL/g;在加入营养液后,经驯化后的活性污泥外源菌分别对甘肃煤和内蒙古煤生物降解的产气实验均出现了产气高峰提前、产气速率提高、产甲烷总量增加的现象,说明营养液的加入对煤生物降解产气产生了促进作用,经驯化后的活性污泥外源菌分别对甘肃煤和内蒙古煤生物降解的产气规律相似,加入营养液后,甘肃煤产甲烷出现两个高峰期,分别出现在第14天和第41天,发酵罐最高压力达183.58 kPa,最高甲烷体积分数为50.22%,最终产甲烷总量为59.08 L,甘肃煤和内蒙古煤产甲烷率分别为5.91 mL/g和5.90 mL/g;驯化后的活性污泥外源菌较本源菌对甘肃煤和内蒙古煤降解产甲烷效果更好,且外源菌在煤生物降解产甲烷后期,利用CO_(2)还原产甲烷途径较为明显。甘肃地区本源菌对本地区煤生物降解产甲烷能力较差,原因可能为本源菌液中硫酸盐含量较高,硫酸盐还原菌生长成为优势菌种,并对产甲烷�
Using drainage water from coalbed methane wells in Gansu and seam seepage from Inner Mongolia as the native bacteria, and activated sludge as the exogenous bacteria, the experiments for biodegradation of coal from the two regions were carried out in a 190 L large fermenter. The fermenter pressure, volume fraction of methane, total methane production and gas production rule, as well as the metabolic pathway of coal methane production, were analyzed during the coal degradation by two kinds of bacteria. The results show that during the biodegradation of Gansu coal after domestication, the maximum pressure of the fermenter reaches 45.8 kPa on the 32 nd day with volume fraction of methane of 30.57% and a total methane production of 8.97 L. After the addition of nutrient solution, the maximum pressure reaches 121 kPa on the 15 th day with volume fraction of methane of 38.22%, a total methane production of 29.55 L, and a methane production rate of 2.95 mL/g. During the biodegradation of Inner Mongolia coal after domestication, the maximum pressure of the fermentation tank reaches 70.56 kPa on the 21 st day with volume fraction of methane of 32.78% and a total methane production of 14.89 L. After the addition of nutrient solution, the maximum pressure reaches 140.73 kPa on the 14 th day with volume fraction of methane of 40.02%, a total methane production of 36.10 L and a methane production rate of 3.61 mL/g. After the addition of nutrient solution, the exogenous bacteria in the acclimated activated sludge also moves up the peak of gas production, increases the gas production rate and the total amount of methane production in the tests of biodegradation gas production from Gansu coal and Inner Mongolia coal, indicating that the addition of nutrient solution has a promotion effect on coal biodegradation gas production. After acclimation, the exogenous bacteria in activated sludge biodegrades Gansu coal and Inner Mongolia coal which produces gas in a similar way. After the addition of nutrient solution, there are two peaks
作者
郭鑫
陈林勇
任恒星
关嘉栋
赵晗
赵娜
GUO Xin;CHEN Linyong;REN Hengxing;GUAN Jiadong;ZHAO Han;ZHAO Na(State Key Laboratory of Coal and CBM Co-mining,048000 Jincheng,China;Yi’an Lanyan Coal and Coal-bed Methane Simultaneous Extraction Technology Company Limited,048000 Jincheng,China)
出处
《煤炭转化》
CAS
CSCD
北大核心
2022年第4期46-54,共9页
Coal Conversion
基金
山西省基础研究项目(2015012001,2016012009)
山西省应用基础研究项目面上青年基金资助项目(201801D221354).
关键词
发酵罐
本源菌
外源菌
活性污泥
产甲烷效果
fermentor
native bacteria
exogenous bacteria
activated sludge
methanogenic effect
