摘要
以绿豆为材料,运用生物信息学、转录组学和酶学等分析方法,对绿豆GST家族基因结构及其表达蛋白的特征进行分析,并检测镉(Cd)胁迫下GST基因表达谱和酶活性变化。结果表明,绿豆基因组中包含有93个GST基因,分为Tau、Phi、Lambda、Theta、Zeta、DHAR、EF1Bg、GHRs、mPGES2和TCHQD等10类,以Phi和Tau类基因数最多;不同类基因结构存在较大差异,Tau类和TCHQD类含有2个外显子,Phi类和GHRs类分别含3和5个外显子,其余基因外显子数目在6~10个。其中只有40个基因在绿豆中表达,31个基因在根、茎、叶中都有表达,6个基因选择性表达。Cd胁迫上调大多数基因的表达量,根、茎、叶中被显著上调的GST基因分别为10、8和4个,平均上调倍数为2.17、2.25和3.26;Cd胁迫显著升高根和茎中GST酶活分别达2.14和1.27倍,与基因表达量的变化一致。表明绿豆根中GST基因及其酶活在Cd胁迫响应中起最主要作用。研究结果为植物GST基因的进一步研究和在植物抗逆中生物技术应用提供了科学资料。
Glutathione transferase(GST)plays an important role in plant response to heavy metal stress.In order to understand the mechanism of GST in mung bean in response to cadmium(Cd)stress,bioinformatics.transcriptomics and enzymatie analysis methods were used to analyze the strueture and expression profile of GST genes,the characteristies of GST proteins,and the changes in GST enzyme activity of mung bean seedlings under Cd treatment or no Cd treatment.The results showed that the genome of mung bean contained 93 GST genes,which were elassified into 10 classes,namely Tau,Phi,Lambda,Theta,Zeta,DHAR,EF1Bg,GHRs,mPGES2 and TCHQD.The Phi and Tau elasses contained the largest number of the genes.There were great difference in gene strue ture of different classes.The Tau and TCHQD elasses contained two exons,the Phi and GHRs clas-ses contained 3 and 5 exons,respectively,and the other classes contained 6 to 10 exons.Among 93;GSH genes,40 were expressed in roots,stems or leaves,of which 31 were expressed in roots,stems,and leaves,and 6 were selectively expressed in the three organs.The majority of the genes were up-regulated by Cd.There were 10,8.and 4 Benes that were significantly up regulated by an average of 2.17,2.25,and 3.26 fold in roots,stems,and leaves,respetively.Cd stress significantly increased GST activity in roots and stems by 2.14 and 1.27 times on average.respectively,and the change trend in GST activity were consistent with that of gene expression.The results suggested that root:GST genes would play a key role in mung bean seedlings in response to Cd stress.The results provide scientific data for the further study of plant GST gene and the biotechnologieal aplication in plant stress resistance.
作者
张创娟
程斌
杨乐
冷艳
李师翁
ZHANG Chuangjuan;CHENG Bin;YANG Le;LENG Yan;LI Shiweng(School of Biological and Pharmaceutical Engineering,Lanzhou Jiaotong University,Lanzhou 730070,China)
出处
《西北农业学报》
CAS
CSCD
北大核心
2022年第6期703-717,共15页
Acta Agriculturae Boreali-occidentalis Sinica
基金
国家自然科学基金(31760110)。
关键词
绿豆
谷胱甘肽转移酶
基因家族
镉胁迫
Vigna radiata(L.)R.Wilczek
Glutathione transferase(GST)
Gene family
Cadmium stress
