摘要
为获悉玉米SEs基因家族的功能和系统进化关系,利用生物信息学方法对玉米SEs基因家族进行鉴定,同时分析其家族成员理化性质、蛋白质二级结构、进化关系、基因结构、保守结构、氨基酸序列、染色体及启动子顺式作用元件。结果表明,在玉米中共鉴定到10个SEs基因家族成员,编码蛋白质的氨基酸序列长度为320~534 aa,分子量大小为33.85~56.94 kDa,等电点为6.57~9.15;亚细胞定位预测结果表明大部分玉米SEs蛋白定位在细胞质和原生质;系统进化树将玉米SEs基因家族分为3支;基因结构的保守基序分析说明玉米SEs基因家族存在一定的差异性;10个玉米SEs基因家族成员主要分布在Chr1、Chr5和Chr9上;氨基酸多序列比对分析表明10个玉米SEs基因家族成员存在一定的结构相似性;蛋白序列中存在10种Motif;启动子顺式作用元件分析表明玉米SEs基因主要受光响应、茉莉酸信号响应调控。
In order to learn the function and phylogenetic relationship of SEs gene family in maize,bioinformatics methods were used to identify the maize SEs gene family,and the physicochemical properties,protein secondary structure,evolutionary relationship,gene structure,conserved structure,amino acid sequence,chromosomal localization and promoter.The results showed that a total of 10 members of the maize SEs gene family were identified in maize.The protein-encoding amino acid sequences ranged in length from 320 to 534,the molecular weight ranged from 33.85 to 56.94 kDa,and the isoelectric points varied in the range of 6.57 to 9.15.The results of subcellular localization prediction showed that most of the maize SEs proteins were located in the cytoplasm and protoplasm,the phylogenetic tree divided the maize SEs gene family into three branches,the conserved motif analysis of the gene structure showed that there were certain differences in the maize SEs gene family.The members of the maize SEs gene family were mainly distributed on Chr1,Chr5 and Chr9,amino acid multiple sequence alignment analysis showed that 10 maize SEs gene family members had certain similarity,there were 10 Motifs in protein sequence,promoter cis-acting element analysis showed that maize SEs genes are mainly regulated by light response and jasmonic acid signal response.
作者
吴立仁
张冬雪
唐贵
隋冬华
武新娟
高佳缘
王腾
王璐瑶
WU Li-ren;ZHANG Dong-xue;TANG Gui;SUI Dong-hua;WU Xin-juan;GAO Jia-yuan;WANG Teng;WANG Lu-yao(Institute of Economic Crops,Heilongjiang Academy of Agricultural Sciences,Harbin 150086,China;Institute of Rural Revitalization Science and Technology,Heilongjiang Academy of Agricultural Sciences,Harbin 150023,China)
出处
《黑龙江农业科学》
2022年第7期14-19,共6页
Heilongjiang Agricultural Sciences
基金
黑龙江省农业科学院“农业科技创新跨越工程”玉米科技创新专项(HNK2019CX03)。
