摘要
目的:建立基于纳米氧化石墨烯的盐酸小檗碱温敏水凝胶(BH-NGO-gel)中盐酸小檗碱及其体外释放度的测定方法。方法:采用高效液相色谱法,C_(18)色谱柱(4.6 mm×250 mm,5µm);流动相乙腈-0.03 mol/L磷酸二氢钾(28∶72,v/v);检测波长350 nm;流速1 ml/min;柱温30℃;进样量10µl。释放介质为含0.5%吐温80的0.1 mol/L磷酸盐缓冲液(PBS,pH 7.4),采用透析法分别于37℃和42℃条件下进行体外释放度试验,并采用DDSolver软件拟合药物释放模型。结果:盐酸小檗碱的线性范围为2~20µg/ml,高、中、低3个浓度的回收率均>95%,RSD均<2%。相比于盐酸小檗碱溶液,BH-NGO-gel释药速度明显较慢(P<0.001);42℃释药速率高于37℃,但无明显统计学差异(P>0.05)。BH-NGO-gel在37℃和42℃下的释药过程均符合Makoid-Banakar释药模型,相关系数r分别为0.9936和0.9869。结论:建立的BH-NGO-gel中盐酸小檗碱体外释放度测定方法简便、可行。BH-NGO-gel具有明显的缓释作用,且具有温度依赖性的释药特征。
Objective:To establish a determination method for berberine hydrochloride and its in vitro release of berberine hydrochloride thermosensitive hydrogel based on nano graphene oxide (BH-NGO-gel).Methods:The high performance liquid chromatography method was used,performed on a C;column(4.6 mm×250 mm,5μm)at a flow rate of 1 ml/min with mobile phase of acetonitrile-0.03 mol/L monobasic potassium phosphate(28∶72,v/v).The detection wavelength was 350 nm,the column temperature was30℃and the injection volume was 10μl.The release medium was 0.1 mol/L phosphate buffer(PBS,pH 7.4)containing 0.5%Tween 80.The in vitro release tests were carried out by dialysis method at 37℃and 42℃respectively.And the kinetic model of drug release was analyzed by DDSolver software.Results:The linear range of berberine hydrochloride was 2~20μg/ml,and the recoveries of high,medium and low concentrations were all more than 95%,and RSD were all less than 2%.Compared with berberine hydrochloride solution,the drug release rate of BH-NGO-gel was significantly slower(P<0.001),and the drug release rate at 42℃was higher than that of 37℃,but there was no significant statistical difference(P>0.05).The in vitro release processes of BH-NGO-gel at 42℃and 37℃were all accorded with Makoid-Banakar model,and the correlation coefficients r was 0.993 6 and 0.986 9,respectively.Conclusion:The established method for determining berberine hydrochloride in vitro release from BH-NGO-gel is simple and feasible.BH-NGO-gel had obvious sustained release effect and temperature dependent drug release characteristics.
作者
直炜炜
王华华
王莎莎
周宁
贾永艳
祝侠丽
ZHI Weiwei;WANG Huahua;WANG Shasha;ZHOU Ning;JIA Yongyan;ZHU Xiali(School of Pharmacy,Henan University of Chinese Medicine,Zhengzhou 450046,Henan,China)
出处
《上海中医药大学学报》
CAS
2022年第3期66-71,共6页
Academic Journal of Shanghai University of Traditional Chinese Medicine
基金
国家自然科学基金资助项目(81803740)
河南省研究生教学改革与质量工程提升项目(YJS2021AL058)。
关键词
盐酸小檗碱
温敏水凝胶
纳米氧化石墨烯
体外释放度
高效液相色谱法
berberine hydrochloride
thermosensitive hydrogel
nano graphene oxide
in vitro release
high performance liquidchromatography
