摘要
为了探究重金属镉和低pH胁迫对中华绒螯蟹抗氧化能力的影响,实验设置了2个pH(7.8、6.5)、3个镉浓度组(0、1.45、2.90mg/L)和3个处理时间(7d、14d、21d),研究镉和低pH胁迫对中华绒螯蟹血细胞总数(THC)、血清中超氧化物歧化酶(SOD)活性、过氧化氢酶(CAT)活性、总抗氧化能力(T-AOC)、丙二醛(MDA)含量以及肝胰腺中谷胱甘肽过氧化物酶(GPx)、谷胱甘肽-s-转移酶(GST)、热休克蛋白70(HSP70)和溶菌酶(LZM)基因4种免疫相关基因mRNA表达水平的影响。结果显示,随镉浓度升高THC显著下降,且在同浓度下pH6.5时THC均低于pH7.8。pH为7.8时,随着镉浓度的升高中华绒螯蟹血清SOD活性和T-AOC均先升后降;而CAT活性(除了第7天)和MDA含量逐渐升高。pH为6.5时,镉浓度2.9mg/L胁迫21d时,SOD、CAT活性和T-AOC均显著低于pH7.8(P<0.05)。在整个实验过程中,镉浓度2.9mg/L、pH 6.5时MDA含量均显著高于pH7.8(P<0.05)。pH为7.8时,GPx、GST、HSP70、LZMmRNA表达量均随着镉浓度升高逐渐升高,镉浓度2.9mg/L胁迫21d时,各基因表达量都达到最高。在14、21d时,镉浓度2.9 mg/L、pH6.5时GPx、GSTmRNA表达量均显著降低,并显著低于同浓度pH7.8表达量(P<0.05);而HSP70、LZMmRNA表达量在21d时显著降低,也显著低于同浓度pH7.8表达量(P<0.05)。综上,抗氧化酶活性以及相关基因mRNA表达量在低pH和镉应激刺激下呈上调或下调趋势以应对氧化应激,低pH和镉协同处理对中华绒螯蟹的抗氧化防御的影响显著大于单一因素的作用,高浓度镉(2.9mg/L)处理的效果更显著。该研究结果为以后研究水体低pH和重金属污染对甲壳类动物影响提供了理论依据。
To investigate the effects of cadmium(Cd)and low pH stress on antioxidant defense ability of the Chinese Mitten Crab(Eriocheir sinensis),two experimental groups with different pH values(7.8,6.5),and three experimental groups with different Cd;concentrations(0 mg/L,1.45 mg/L and 2.9 mg/L),were set and exposed for 7 d,14 d,and 21 d,respectively.The effects of cadmium and low pH stress on total hemocyte counts(THC),serum superoxide dismutase(SOD)activity,catalase(CAT)activity,total antioxidant capacity(T-AOC),malondialdehyde(MDA)content and four immune related genes(glutathione peroxidase(GPx),glutathione S-transferase(GST),heat shock protein 70(HSP70),lysozyme(LZM)mRNA in hepatopancreas of Eriocheir sinensis were studied.The results showed that THC decreased significantly with the increase of Cd concentration,and THC at pH 6.5 was lower than that at pH 7.8.When the pH value was 7.8,with the increase of cadmium concentration,the activities of SOD and T-AOC in serum increased first and then decreased,while the activities of CAT(except the 7 th day)and the content of MDA increased gradually.The activities of SOD,CAT,and T-AOC were significantly lower than those of pH 7.8(P<0.05).During the whole experiment,the content of MDA at 2.9 mg/L and pH 6.5 was significantly higher than that at pH 7.8(P<0.05).When pH value was 7.8,the mRNA expression levels of GPx,GST,HSP70 and LZM increased gradually with the increase of cadmium concentration.When cadmium concentration was 2.9 mg/L for 21 days,the gene expression levels reached the highest.On the 14 th and 21 st day,the expression of GPx and GST mRNA at 2.9 mg/L and pH 6.5 of cadmium was significantly decreased,which was significantly lower than that at pH 7.8 of the same concentration(P<0.05),while the expression of HSP70 and LZM mRNA was significantly decreased only on the 21 st day,which was also significantly lower than that at pH 7.8 of the same concentration(P<0.05).In general,the activity and content of antioxidant enzymes and the mRNA expression of related genes were u
作者
杨志刚
赵雪健
成永旭
YANG Zhigang;ZHAO Xuejian;CHENG Yongxu(Key Laboratory of Freshwater Aquatic Genetic Resources,Ministry of Agriculture and Rural Affairs,Shanghai Ocean Universityy Shanghai 201306,China;Centre for Research on Environmental Ecology and Fish Nutrition,Ministry of Agriculture and Rural Affairs,Shanghai Ocean University,Shanghai 201306,China;National Demonstration Center for Experimental Fisheries Science Education,Shanghai Ocean University,Shanghai 201306,China)
出处
《上海海洋大学学报》
CAS
CSCD
北大核心
2022年第2期373-383,共11页
Journal of Shanghai Ocean University
基金
国家重点研究发展计划(2018YFD0900603)
现代农业产业技术体系专项资金资助(CARS-48)
上海市科学技术委员会工程技术中心能力提升项目(19DZ2284300)
黄河三角洲人才工程(DYRC20190210)。
关键词
中华绒螯蟹
镉
pH
抗氧化酶活性
免疫基因
Eriocheir sinensis
cadmium
pH
antioxidant enzyme activity
immune gene
