摘要
目的探讨乙酰辅酶A羧化酶1(ACC1)对人胶质瘤细胞系U87细胞增殖、迁移及侵袭的作用。方法Western blotting检测人胶质瘤细胞系U87、U251及U373中ACC1的表达;构建ACC1过表达质粒载体,将过表达ACC1质粒载体瞬时转染至U87细胞中;Western blotting检测转染后U87细胞中ACC1表达情况;MTT实验检测过表达ACC1对U87细胞增殖的影响;Transwell迁移和侵袭实验分别检测过表达ACC1对U87细胞迁移和侵袭的影响;划痕实验检测过表达ACC1对U87细胞划痕愈合能力的影响;Western blotting检测相关蛋白表达变化。结果与人胶质瘤细胞系U251和U373相比,U87细胞中ACC1表达较低;ACC1过表达抑制U87细胞增殖(P<0.01);ACC1过表达抑制U87细胞迁移、侵袭和划痕愈合能力(P<0.01);ACC1过表达迁移和侵袭相关蛋白波形蛋白(vimentin)、纤维连接蛋白(fibronectin)和尿激酶型纤溶酶原激活剂(uPA)表达下调(P<0.01),凋亡抑制蛋白Bcl-2和细胞周期蛋白(cyclin)B、cyclin D表达下调(P<0.01),p-STAT3蛋白表达下调(P<0.01),细胞周期蛋白P21表达上调(P<0.01)。结论过表达ACC1可能通过抑制STAT3活性,抑制人胶质瘤细胞的增殖、迁移和侵袭。
Objective To explore the effect of acetyl-CoA carboxylase 1(ACC1)on cell proliferation,migration and invasion of human glioma cell line U87.Methods Western blotting was performed to examine endogenous ACC1 expression in human glioma cell lines U87,U251 and U373.ACC1 overexpression plasmid and the plasmid vector were transiently transfected into U87 cells.The level of ACC1 in control and ACC1 overexpression cells was examined by Western blotting.The effect of ACC1 on U87 cells migration and invasion was detected by Transwell assay.The effect of ACC1 on U87 cells scratch healing ability was detected by scratch test.The effect of ACC1 on U87 cells proliferation was investigated by MTT assay.Western blotting was conducted to detect the level changes of proteins.Results Among three human glioma cell lines U87,U251 and U373,endogenous ACC1 level in U87 cells was lower than that in other two cell lines.ACC1 overexpression inhibited U87 cell proliferation,as well as cell migration,invasion and scratch healing ability(P<0.05).Vimentin,fibronectin,urokinase type plasminogen activator(uPA),Bcl-2,cyclin B,cyclin D and p-STAT3 were down-regulated(P<0.05),P21 was up-regulated(P<0.05)after ACC1 overexpression.Conclusion These results suggest that ACC1 suppresses the proliferation,migration and invasion of human glioma cells,probably by inhibiting STAT3 activity.
作者
王阳
赵宝生
李超红
刘玉珍
WANG Yang;ZHAO Bao-sheng;LI Chao-hong;LIU Yu-zhen(Life Science Research Center,the First Affiliated Hospital of Xinxiang Medical University,He'nan Weihui 453100,China;Department of Thoracic Surgery,the First Affiliated Hospital of Xinxiang Medical University,He'nan Weihui 453100,China)
出处
《解剖学报》
CAS
CSCD
北大核心
2022年第3期317-322,共6页
Acta Anatomica Sinica
基金
河南省神经修复重点实验室开放课题(HNSJXF-2021-014)
河南省科技厅科技攻关计划项目(202102310103)。
