摘要
将待保存菌株接种在PDA培养基平板中央,在菌落周围放置直径3~5 mm的圆形无菌滤纸片,待真菌菌丝(孢子)长满滤纸片后,将其取出放入无菌培养皿中,放置在底部盛有变色硅胶的干燥器中干燥3~5 d。将干燥后的带菌滤纸片再转入底部装有无菌变色硅胶的无菌离心管中,用封口膜封口,放入透明冷冻盒内-20℃保存。通过对保存周期为2~7年的15种真菌菌种共计260个菌株的存活情况及部分菌株的致病性进行检测,发现保存的菌种存活率高,致病性和生物学特性不易退化。因此,滤纸片低温干燥法保存丝状真菌菌种,具有对设备要求简单、占用空间小、保存容量大、保存时间长、工作效率高等优点。
The strains to be preserved were inoculated in the center of the PDA medium plate,a circular sterile filter paper with a diameter of 3 to 5 mm was placed around the colony.After the fungal hyphae(spores)were overgrown with the filter paper,took it out and put it into a sterile petri dish,and placed it in a desiccator filled with discolored silica gel at the bottom to dry for 3 to 5 days.Dried filter paper pieces were then put into centrifuge tubes with sterile silica gel at the bottom.After sealing with parafilm,the centrifuge tubes were finally put into a transparent freezer box and stored at-20℃.Through the detection of 260 isolates from 15 fungi species that preserved for 2-7 years,it was found that most of the detected strains maintained high survival rate and high virulence,and their biological characteristics do not degenerate easily.Using drying filter paper at low temperature to save filamentous fungi has the advantages such as requires simple equipments,occupies small space,has large storage capacity,keeps long storage time,and has high efficiency.
作者
尹良芬
YIN Liang-fen(Experimental and Teaching Center of Crop Science in College of Plant Science and Technology,Huazhong Agricultural University,Wuhan,Hubei 430070)
出处
《安徽农业科学》
CAS
2022年第12期5-10,共6页
Journal of Anhui Agricultural Sciences
关键词
菌种保存
滤纸片干燥
低温
存活率
致病性
丝状真菌
Fungi strain conservation
Drying filter paper pieces
Low temperature
Survival rate
Pathogenicity
Filamentous fungi
