摘要
[目的]研究盐浓度对水稻土壤固氮微生物nifH基因多样性的影响。[方法]在不同盐浓度的土壤区域采集样品,提取土壤总DNA,采用末端限制性片段长度多样性分析技术(T-RFLP)对土壤固氮微生物nifH基因进行多样性分析。[结果]随着水稻土壤盐度上升,土壤有机质、全氮和碱解氮含量也呈现一定程度的上升趋势;水稻土壤nifH基因的分类操作单元(OTU)总数在土壤盐度最高时达最大值,各组优势细菌数量在2.5种左右,样本丰度主要集中在4个片段内,各组相对丰度差异不显著(P>0.05);水稻组土壤与富贵竹土壤相比,多样性和均匀度均差异显著(P<0.05),水稻各组间微生物多样性呈现盐度依赖效应,但差异不显著(P>0.05)。[结论]土壤盐度影响土壤性质及固氮微生物,微生物多样性呈现一定盐度依赖效应。
[Objective]To study the effect of salt concentration on the nifH gene diversity of nitrogen-fixing microorganisms in rice soil.[Method]Soil samples were collected from different salt concentrations parts and extracted soil total DNA to investigate effects of salt concentration on nifH gene diversity of rice soil nitrogen-fixing microorganisms by using the terminal restriction fragment length polymorphism(T-RFLP)methods.[Result]The content of organic matter,total nitrogen and alkali-hydrolyzable nitrogen also showed a certain upward trend with the increase of soil salinity.The total number of operational taxonomic units(OTUs)of nifH gene in rice soil reached the maximum value at the highest soil salinity,the number of dominant bacteria was about 2.5 in each group,and the sample abundance was mainly concentrated in 4 fragments.There was no significant difference in relative abundance among the groups(P>0.05)The diversity and evenness of rice soil were significantly different from those of Fugui Bamboo,there was a salinity-dependent effect on microbial diversity among rice groups,but the differences were not significant(P>0.05).[Conclusion]Soil salinity affected properties and nitrogen-fixing microorganisms,and microbial diversity showed a salinity-dependent effect.
作者
廖洁深
何杰珩
刘广超
马驿
丁月霞
周鸿凯
LIAO Jie-shen;HE Jie-heng;LIU Guang-chao(College of Coastal Agricultural Sciences,Guangdong Ocean University,Zhanjiang,Guangdong 524088)
出处
《安徽农业科学》
CAS
2022年第12期1-4,共4页
Journal of Anhui Agricultural Sciences
基金
广东省重点领域研发计划项目(2020B020219004)
广东海洋大学大学生创新项目(CXXL2020190)
广东省教育厅2021年度普通高校重点领域专项(2021ZDZX4003)
湛江市科技局2021年度省科技专项(2021A05231)。
