摘要
【目的】为探究香蕉中蛋白质精氨酸甲基转移酶(protein arginine methyltransferases,PRMT)的蛋白功能特征、表达模式以及植物PRMT5的进化关系,以便从分子和基因层面揭示香蕉抗逆机理。【方法】利用生物信息学方法对香蕉A(Musa acuminata)、香蕉B(Musa balbisiana)和阿宽蕉(Musa itinerans)PRMT家族进行全基因组鉴定,对其蛋白质理化性质、系统进化树、保守基序、蛋白结构域、启动子响应元件预测、蛋白互作、共线性和低温转录组表达模式进行系统分析,并对30种植物的PRMT5基因进行进化分析。【结果】3种香蕉中均存在8个PRMT家族成员,蛋白结构域和精氨酸甲基化相关,亚细胞定位主要在叶绿体和细胞膜上。系统进化树显示香蕉PRMT分为5个亚族,且与拟南芥和水稻PRMT同源性高;启动子中含有光响应、逆境胁迫响应和植物激素响应元件。香蕉物种内共线性分析结果显示,基因复制事件分别发生1、2、0次,与拟南芥进行物种间共线性分析证明,香蕉A和B之间亲缘关系最近。低温转录组表明香蕉PRMT成员中呈现出两大类不同的表达模式:第Ⅰ类大致在0℃和4℃时表达量高,说明低温促进第Ⅰ类基因表达;第Ⅱ类基因中MiPRMT5、MiPRMT7、MiPRMT3、MiPRMT8和MaPRMT2这5个基因在13℃时表达量最高,其余温度下表达量较低或不表达。PRMT5进化分析结果表明,同科植物在进化过程中基因功能多样化表达,该基因兼具多样性与保守性。【结论】PRMT在香蕉中参与精氨酸甲基化和DNA损伤修复,参与非生物胁迫并响应脱落酸、茉莉酸甲酯、水杨酸等物质的代谢通路,从而发挥逆境防御等功能。结合低温转录组数据,表明不同梯度的低温可能会调控不同的PRMT基因表达,使其适应多种冷胁迫条件,提高自身抗逆性。PRMT5进化分析结果显示,香蕉PRMT5在进化过程中相对独立,与其他植物亲缘关系较远,推测香蕉中该基因编码蛋�
[Objective]This study aims to explore the protein functional characteristics,expression patterns of the protein arginine methytransferases(PRMT)and the evolutionary relationships of PRMT5,through which the stress resistance mechanism of bananas at the molecular and genetic levels can be revealed.[Methods]Bioinformatics methods were used to implement genome wide identification of the PRMT family of banana A(Musa acuminata),banana B(Musa balbisiana)and Musa itinerans.The system analysis was conducted to investigate the physical and chemical properties of protein,the phylogenetic tree,conservative motif,protein domains,promoter response element predictions,protein-protein interactions as well as expression patterns and collinearity of low temperature transcriptome.In addition,PRMT5 genes of 30 sorts of plants have been put into evolutionary analysis in detail.[Results]The results indicated that all three kinds of bananas contain 8 members of PRMT family.The protein domain was associated with arginine methylation and subcellular localization was mainly in chloroplast and membrane.The phylogenetic tree indicated that the PRMTs of bananas consisted of five subgroups and they had high homology with the PRMTs of Arabidopsis thaliana and the PRMTs of Oryza sativa L.In addition,The promoter of the PRMTs of bananas contained light response,stress response and plant hormone response elements.Intraspecific collinearity analysis of banana showed that gene duplication events occurred 1,2 and 0 times,respectively.Interspecific collinearity analysis showed that banana A and B had the closest genetic relationship with Arabidopsis thaliana.Cryotranscriptome analysis showed that there were two different expression patterns in PRMT members of banana:the first type had the highest expression level at approximately 0℃and 4℃,which indicated that the low temperature promoted the expression of the first type,while the second type of genes,which included 5 genes:MiPRMT5,MiPRMT7,MiPRMT3,MiPRMT8 and MaPRMT2,had the highest expression lev
作者
范正阳
吴秋桢
张春渝
许小琼
李丹
孙莹
刘生财
陈裕坤
林玉玲
赖钟雄
FAN Zhengyang;WU QiuZhen;ZHANG Chunyu;XU Xiaoqiong;LI Dan;SUN Ying;LIU Shengcai;CHEN Yukun;LIN Yuling;LAI Zhongxiong(Institute of Horticultural Plant Bioengineering,Fujian Agriculture and Forestry University,Fuzhou 350002,China)
出处
《江西农业大学学报》
CAS
CSCD
北大核心
2022年第3期569-582,共14页
Acta Agriculturae Universitatis Jiangxiensis
基金
国家重点研发计划项目(2019YFD1000901)
国家现代农业产业技术体系(香蕉)专项资金(GARS-31-15)。