摘要
为开展西藏自治区绒山羊腹腔镜胚胎移植研究工作,2020年8月23日,于西藏自治区农牧科学院畜牧兽医研究所实验室内开羊胚胎移植试验。本次试验选用健康经产空怀供体羊6只,其中藏西北紫绒山羊4只,高产藏西北白绒山羊2只;受体羊为32只健康经产空怀藏西北白绒山羊。经供体超数排卵和供、受体羊同期发情处理后,供体使用常规手术方法收集胚胎,受体使用腹腔镜技术进行胚胎移植,于术后45 d对移植受体进行B超妊娠诊断。试验结果表明,6只供体羊均超数排卵,共收集56枚可用胚胎,超排率100%(6/6),其中最少黄体个数为4,最多黄体个数为23,平均有效胚胎为9.3枚/只;受体发情29只,受体发情率为90.6%(29/32),移植29只,移植率为100%(29/29),其中27只移植双胚,2只移植单胚;B超妊娠诊断中,妊娠羊13只,受胎率为44.8%(13/29);产羔统计中,共8只受体生产9只羔羊,产羔率112.5%(9/8),羔羊成活8只,成活率88.9%(8/9)。本研究系西藏自治区首次使用腹腔镜开展藏西北绒山羊胚胎移植试验,对于高原地区绒山羊高效繁殖技术的研究应用具有重要参考意义。
In order to make research on laparoscopic embryo transfer of cashmere goats in Tibet Autonomous Region,embryo transfer experiment was conducted in the laboratory of Animal Husbandry and Veterinary Research Institute of Tibet Academy of Agricultural and Animal Husbandry Sciences on August 23,2020.We selected 6 healthy empty pregnant goats,including 4 Tibetan northwest purple cashmere goats and 2 high-yield Tibetan northwest white cashmere goats.The receptors were 32 healthy white cashmere goats.After superovulation of the donor and simultaneous estrus of the donor and receptors,embryos were collected by the donor using conventional surgical method,and embryos were transplanted by the receptor using laparoscopic technique.B-ultrasound diagnosis of pregnancy was performed on the receptor 45 days after operation.The results showed that all the six goats were superovulated,and a total of 56 available embryos were collected,with a 100%(6/6)superovulation rate.The minimum number of luteal cells was 4,the maximum number of luteal cells was 23,and the average number of available embryos was 9.3.The estrus rate of receptors was 90.6%(29/32),and the transfer rate was 100%(29/29).Among them,27 were double embryos and 2 were single embryos.In B-ultrasound pregnancy diagnosis,there were 13 pregnant goats,and the conception rate was 44.8%(13/29).In lambing statistics,a total of 8 receptors produced 9 lambs and the lambing rate was 112.5%(9/8),among them,the survival rate was 88.9%(8/9).This study is the first experiment of embryo transfer of Tibetan Northwest Cashmere Goats by laparoscopy in Tibet Autonomous Region,which has important reference significance for the research and application of efficient breeding technology of cashmere goat in plateau region.
作者
巴贵
BA Gui(Institute of Animal Husbandry and Veterinary Medicine,Tibet Academy of Agricultural and Animal Husbandry Sciences,Lhasa Tibet 850009,China)
出处
《畜牧兽医杂志》
2022年第4期15-18,共4页
Journal of Animal Science and Veterinary Medicine
基金
西藏自治区科技计划-中央引导地方项目(XZ202001YD0017C)
西藏十四五重大科技专项"西藏羊品种选育与健康养殖"(XZ202101ZD0001N)
国家现代农业产业技术体系资助(CARS-39-32)。
关键词
藏西北紫绒山羊
腹腔镜
胚胎移植
Tibet northwest purple cashmere goats
laparoscopy
embryo transfer
