摘要
转移核糖核酸(tRNA)反密码子环上存在大量的转录后修饰,这些修饰对于tRNA的稳定、三级结构维持、氨基酰化能力以及密码子解码速度与保真性等有重要作用。近年来,随着对tRNA修饰研究的深入,越来越多的研究结果表明,tRNA修饰与疾病密切相关。tRNA第37位(反密码子后第1位)是高度保守的修饰位点,缺失tRNA第37位修饰会影响细胞生长和能量代谢,最终导致疾病发生。尽管人tRNA第37位修饰的类型已经明确,但是某些修饰酶及/或辅助因子尚未被鉴定,且若干修饰的催化机制和功能还不清晰。该文就目前已经报道的人tRNA第37位修饰的种类和功能作简要总结,以期为后续深入开展tRNA第37位修饰的研究和深入认识该位点修饰缺陷导致疾病的分子机制提供思路。
Anti-codon loop of transfer RNAs(tRNAs)harbors the most diverse post-transcriptional modifications,playing important roles in t RNA stability,tertiary structure maintenance,aminoacylation and genetic code decoding.With the in-depth study of tRNA modification,increasing recent results have shown that tRNA modifications are firmly related to diseases.Position 37 of tRNA(the first position after the anticodon)is a highly conserved modified site.Hypomodifications of tRNA at position 37 affect cell growth and energy metabolism,eventually leading to diseases.Although the modification types of human tRNA at position 37 have been identified,some modifying enzymes and/or cofactors have not been discovered,and the catalytic mechanism and function of many modifications are not fully understood.In this review,we briefly summarize the recent advances in human tRNA modifications at position 37 in order to have an in-depth understanding of tRNA modifications at this position and provide insights into the molecular mechanism of diseases caused by the deficiency of tRNA modifications at position 37.
作者
周敬波
周小龙
王恩多
ZHOU Jingbo;ZHOU Xiaolong;WANG Enduo(Center for Excellence in Molecular Cell Science,Chinese Academy of Sciences,Institute of Biochemistry and Cell Biology,Chinese Academy of Sciences,Shanghai 200031,China;University of Chinese Academy of Sciences,Beijing 100864,China;School of Life Science and Technology,ShanghaiTech University,Shanghai 201210,China)
出处
《生命的化学》
CAS
2022年第2期159-166,共8页
Chemistry of Life
基金
国家自然科学基金项目(91940302,31870811,31822015,81870896)。
关键词
TRNA
tRNA修饰
翻译
移码
tRNA
tRNA modification
translation
frameshifting
