摘要
Multimodal therapy has been recognized as a powerful platform for the precise treatment of cancer.However,the reported multimodal therapeutic systems often involve sophisticated components and tedious fabrication procedures.As such,developing nature-inspired and easilyobtainable theranostic agents for multimodal cancer therapy remains challenging.In this work,we propose nature-inspired nanothylakoids as a multimodal theranostic agent for cancer therapy both in vitro and in vivo.Nanothylakoids extracted from spinach leaves exhibit prominent photothermal and photodynamic inactivation on 4T1 and MCF-7 cancer cells due to their outstanding photothermal conversion/photosensitization capabilities.Additionally,the peroxidase-like catalytic activity of nanothylakoids is simultaneously verified,which facilitates the oxidation of H_(2)O_(2)to the cytotoxic hydroxyl radical(·OH)and thus cause efficient cell apoptosis.Interestingly,a selective cytotoxicity of nanothylakoids on MCF-7 cancer cells is found due to their overexpression of H_(2)O_(2).In vitro and in vivo results substantiate the prominent therapeutic outcome and excellent biosafety of nanothylakoids.Nanothylakoids with photothermal/photodynamic effects and peroxidase-like catalytic activity open a new avenue for the development of nature-inspired theranostic materials,holding great promise in multimodal cancer therapeutics.
多模式联合治疗已成为抗肿瘤的有效手段,但目前已报道的多模式肿瘤治疗试剂通常需要复杂的功能组分和繁琐的合成过程.因此,开发一种简单制备的天然来源多模式肿瘤治疗试剂仍然是一个挑战.本文从菠菜叶片中提取出了一种天然来源的、具有类酶性质的纳米类囊体用于肿瘤的多模式治疗.结果表明,纳米类囊体具有良好的光热转换和光敏化性质,可以有效杀伤4T1和MCF-7肿瘤细胞.同时,该材料还具有类过氧化物酶的性质,可以氧化分解过氧化氢产生羟基自由基从而诱导肿瘤细胞凋亡.因MCF-7肿瘤细胞内过量表达过氧化氢,该材料可实现对MCF-7肿瘤细胞的选择性杀伤.该纳米类囊体在细胞水平和活体小动物水平对肿瘤具有显著的治疗效果,且表现出良好的生物安全性.研究表明,该纳米类囊体具有光热转换、光敏化、类过氧化物酶的性质,是一类很有应用前景的多模式肿瘤治疗试剂.
基金
supported by the National Natural Science Foundation of China(22021002,22020102005,and 22022705)
the Strategic Priority Research Program of the Chinese Academy of Sciences(XDA16020804)。
