摘要
目的探讨胃癌患者微小RNA(miRNA)-21和miRNA-335-5p表达与胃蛋白酶原(PG)和胃泌素17(G-17)的相关性。方法选择临海市第二人民医院2019年1月至2021年1月收治的胃癌患者61例为研究对象,选择同期该院健康体检者60例作为对照组。采用乳胶增强免疫比浊法测定PGⅠ、PGⅡ和G-17水平;采用qRT-PCR法测定miRNA-21和miRNA-335-5p表达。比较两组PGⅠ、PGⅡ和G-17水平,miRNA-21和miRNA-335-5p表达;miRNA-21和miRNA-335-5p诊断胃癌灵敏度和特异度;分析miRNA-21和miRNA-335-5p与PGⅠ、PGⅡ和G-17的相关性。结果胃癌组血清PGⅠ[(54.36±9.89)μg/L]、G-17[(13.74±1.89)pg/mL]均低于对照组的(112.31±23.24)μg/L和(18.75±2.36)pg/mL;PGⅡ[(24.35±4.53)μg/L]高于对照组的(20.37±3.28)μg/L,两组差异均有统计学意义(t=17.89、12.90、5.52,均P<0.05)。胃癌组miRNA-21 mRNA相对表达量[(3.42±0.61)]高于对照组的(0.53±0.12);miRNA-335-5p mRNA相对表达量[(0.32±0.17)]低于对照组的(1.65±0.35),两组差异均有统计学意义(t=30.01、26.65,均P<0.05)。受试者工作特征(ROC)曲线分析显示,miRNA-21诊断胃癌灵敏度为74.36%,特异度为68.18%;miRNA-335-5p诊断胃癌灵敏度为79.49%,特异度为60.90%。miRNA-21与PGⅠ和G-17呈线性负相关(r=-0.82、-0.74),而与PGⅡ呈线性正相关(r=0.76);miRNA-335-5p与PGⅠ和G-17呈线性正相关(r=0.79、0.72),而与PGⅡ呈线性负相关(r=-0.70)。结论胃癌患者miRNA-21高表达,而miRNA-335-5p低表达,且与PG和G-17具有明显相关性,可作为诊断胃癌有效指标,研究成果具备显著创新性和科学性。
Objective To correlate the expression of microRNA(miRNA)-21 and miRNA-335-5p with pepsinogen and gastrin-17 in patients with gastric cancer.Methods Sixty-one patients with gastric cancer who received treatment in Linhai Second People's Hospital between January 2019 and January 2021 were included in the patient group.An additional 60 healthy patients who concurrently received physical examination were included in the control group.Serum pepsinogen I,pepsinogen II and gastrin-17 levels were determined by latex-enhanced immunoturbidimetry.miRNA-21 and miRNA-335-5p expression were determined by quantitative reverse transcription-polymerase chain reaction.Serum pepsinogen I,pepsinogen II and gastrin-17 levels and miRNA-21 and miRNA-335-5p expression were compared between the two groups.The sensitivity and specificity of miRNA-21 and miRNA-335-5p in the diagnosis of gastric cancer were analyzed.The correlation between miRNA-21 and miRNA-335-5p expression and pepsinogen I,pepsinogen II and gastrin-17 levels was analyzed.Results Serum pepsinogen I and gastrin-17 levels in the patient group were(54.36±9.89)μg/L and(13.74±1.89)pg/mL,respectively,which were significantly lower than those in the control group[(112.31±23.24)μg/L,(18.75±2.36)pg/mL,t=17.89,12.90,both P<0.05].Serum pepsinogen II level in the patient group was significantly higher than that in the control group[(24.35±4.53)μg/L vs.(20.37±3.28)μg/L,t=5.52,P<0.05].The relative mRNA expression of miRNA-21 in the patient group was significantly higher than that in the control group[(3.42±0.61)vs.(0.53±0.12),t=30.01,P<0.05].The relative mRNA expression of miRNA-335-5p in the patient group was significantly lower than that in the control group[(0.32±0.17)vs.(1.65±0.35),t=26.65,P<0.05].The receiver operating characteristic curve analysis showed that the sensitivity and specificity of miRNA-21 in the diagnosis of gastric cancer were 74.36%and 68.18%,respectively,and they were 79.49%and 60.90%for miRNA-335-5p,respectively.There was a negative linear correlat
作者
陈函清
周初夏
章剑坚
Chen Hanqing;Zhou Chuxia;Zhang Jianjian(Department of Gastroenterology,Linhai Second People's Hospital,Linhai 317016,Zhejiang Province,China)
出处
《中国基层医药》
CAS
2022年第5期664-668,共5页
Chinese Journal of Primary Medicine and Pharmacy
基金
浙江省台州市科技局计划项目(1902KY135)。
