摘要
该研究探讨了黑果腺肋花楸(黑果)果实、叶子的多酚调节抑郁小鼠模型的作用机理,旨在通过饮食干预达到调节抑郁的目的。注射4 mg/mL皮质酮构建小鼠抑郁症模型,采用行为实验、16S rRNA基因测序技术等对小鼠行为、肠道菌群组成与脑源性神经营养因子(brain-derived neurotrophic factor,BDNF)的变化规律等进行研究。研究表明黑果多酚提取物可以显著改善抑郁症小鼠游泳、糖水偏好等行为,可将肠道菌群多样性水平升高至18.23%;使得大脑中BDNF水平可提高81.39%。黑果多酚提取物通过调控肠道菌群、大脑BDNF途径影响抑郁小鼠行为,改善抑郁状态,从而发挥抑郁调节作用,为黑果宽渠道深度开发提供了新支撑,高值化应用提供了理论指导,为饮食调理抑郁症提供新资源,也丰富了功能性食品类型和理论。
To contribute to current research on the regulation of depression through dietary intervention,the anti-depression effects of polyphenol extracts from Aronia melanocarpa fruits and leaves were investigated using a mouse model of this mental health disorder.First,4 mg/mL corticosterone was injected into mice to induce depression in the animals.Then,after administering the polyphenol extracts to the depressed mice,changes in the animals’behavior,intestinal flora,and brain-derived neurotrophic factor(BDNF)level were monitored and analyzed using behavioral experiments,16S rRNA gene sequencing,and other tests.The forced swimming test and sucrose preference test results demonstrated that the polyphenol extracts could significantly improve the behavior of depressed mice.Specifically,the extracts from Aronia melanocarpa leaves outperformed those from the fruits.Comparison between the untreated control group and extract-treated group revealed that the polyphenols had increased the diversity of intestinal flora and the level of BDNF by 18.23%and 81.39%,respectively,suggesting that the polyphenols from Aronia melanocarpa could regulate both the intestinal flora and BDNF to suppress the depression behavior of mice.These findings provide new evidence on the high-value application of Aronia melanocarpa and theoretical support for further in-depth research of the plant,which may give rise to new resources for the dietary intervention of depression and enrich the diversity of and theories behind functional foods.
作者
嵇威
盛桂华
陈炫宏
董雷超
南希骏
张军
周泉城
JI Wei;SHENG Guihua;CHEN Xuanhong;DONG Leichao;NAN Xijun;ZHANG Jun;ZHOU Quancheng(School of Agricultural Engineering and Food Science,Shandong University Key Laboratory of Functional Agricultural Products Technology,Shandong University of Technology,255049 Zibo,China;Zibo Urban and Rural Planning Development Center,255000 Zibo,China)
出处
《现代食品科技》
CAS
北大核心
2022年第6期46-54,353,共10页
Modern Food Science and Technology
基金
山东省重点研发计划项目(2019GNC106084)
山东理工大学招远工业技术研究院创新研究基金(9101-219194,9101-220193)
山东省研究生教育管理项目(117009)
山东理工大学研究生教育创新团队(4053-218049,4053-219076)。
